阿纳迪南特拉科卢布里纳通过抑制NF-κB和p38-MAPK信号通路来调节LPS诱导的炎症
Carolina Medeiros de Almeida Maia1,2, Priscilla Guimarães Silva Vasconcelos1, Silvana Pasetto3
1Department of Dentistry, Postgraduate Program in Dentistry, State University of Paraiba, Campina Grande, Paraiba, Brazil.
Scientific reports
|July 11, 2024
概括
阿纳迪南特拉科卢布里纳提取物通过降低IL-1β和IL-10等关键细胞因子的调节来调节炎症反应. 这种植物提取物影响核因子-卡帕B (NF-κB) 和线粒激活蛋白激酶 (MAPK) 途径,具有低细胞毒性的抗炎作用.
科学领域:
- 植物化学 植物化学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 炎症是一种复杂的生物反应,涉及各种细胞因子和信号通路.
- 阿纳迪南特拉科卢布里纳是一种具有潜在药用功能的植物.
- 了解植物提取物调节免疫反应的分子机制对于开发新疗法至关重要.
研究的目的:
- 为了确定Anadenanthera colubrina标准化提取物的化学特征.
- 为了研究提取物对脂聚糖 (LPS) 诱导的THP-1单细胞中的炎症性细胞因子 (IL-8,IL-1β,IL-10) 和关键信号蛋白 (NF-κB,MAPK) 的作用.
- 为了评估提取物的细胞毒性.
主要方法:
- 高性能液体染色学 (HPLC) 与电子喷射电离质谱学 (ESI-MSn) 和高分辨率电子喷射电离质谱学 (HRESIMS) 结合,用于化学分析.
- 反转录聚合酶链反应 (RT-PCR) 和Luminex检测基因表达和细胞因子水平.
- 简单的西方测定用于检测NF-κB和MAPK通路中的关键蛋白质.
- 细胞活力测试以确定细胞毒性.
主要成果:
- 化学资料显示了类黄,包括儿茶素,奎尔丁,凯姆菲罗尔和益西亚尼丁.
- 阿纳迪南特拉科卢布里娜提取物表现出较低的细胞毒性 (LD50 = 978.7μg/mL).
- 提取物降低了IL-1β和IL-10的基因表达,而IL-1β的细胞因子表达被降低,IL-10在LPS刺激时被上调.
- 提取物在与TLR-4相关的NF-κB/MAPK通路内调节了p38和NF-κB蛋白的酸化,降低了信号强度.
结论:
- 阿纳迪南特拉科卢布里纳标准化提取物具有丰富的黄类蛋白质.
- 提取物通过调节IL-1β和IL-10的表达和分泌而表现出显著的抗炎性质.
- 抗炎作用通过细胞内NF-κB和p38-MAPK信号通路的调节来调节,表明其作为抗炎保护剂的潜力.
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