PPTC7通过通过SCFFBXL4促进BNIP3和NIX降解来对抗菌
Giang Thanh Nguyen-Dien1,2, Brendan Townsend1, Prajakta Gosavi Kulkarni1
1Faculty of Medicine, School of Biomedical Sciences, University of Queensland, Brisbane, QLD, Australia.
EMBO reports
|July 11, 2024
概括
线粒体酸酶PPTC7作为一个关键的适配器,将线粒体受体BNIP3和NIX与SCFFBXL4复合体联系起来. 这种相互作用抑制了过度的线粒体,防止了线粒体疾病.
科学领域:
- 细胞生物学 细胞生物学
- 线粒体生物学 线粒体生物学
- 自学研究 自学研究
背景情况:
- 线粒体的选择性降解,即线粒体的选择性降解,需要严格的调节来保持细胞健康.
- 通常情况下,SCFFBXL4泛素酶复合体通过降解BNIP3和NIX受体来抑制线粒细胞衰变.
- 在FBXL4突变导致线粒体疾病由于不受控制的线粒体.
研究的目的:
- 阐明线粒体酸酶PPTC7在调节线粒体衰变中的作用.
- 调查PPTC7如何与髓受体和SCFFBXL4复合体相互作用.
- 了解PPTC7功能在预防线粒体功能障碍方面的影响.
主要方法:
- 研究了PPTC7,BNIP3,NIX和FBXL4.4之间的相互作用.
- 评估了PPTC7酸酶活性对线粒体受体周转率的影响.
- 映射了参与PPTC7介导复合体形成和线粒细胞衰变抑制的关键残留物.
主要成果:
- PPTC7的功能是作为适配器,而不是通过其酸酶活性,将BNIP3/NIX与FBXL4连接起来进行降解.
- 在线粒体诱导或FBXL4缺失期间,PPTC7在线粒体外膜上积聚,表明反.
- 破坏PPTC7相互作用会损害BNIP3/NIX降解和线粒抑制.
结论:
- PPTC7是SCFFBXL4介导的髓抑制的一个重要辅因子.
- PPTC7作为一个关键的适应蛋白,将菌体受体连接到全方位酸酶复合体.
- 这种涉及PPTC7的调节机制对于预防过度的线粒细胞衰变和维持线粒体平衡至关重要.
相关概念视频
Translocation of Proteins into the Mitochondria
3.1K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.1K
Mitochondrial Protein Sorting
4.3K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death. Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.3K
Electron Transport Chain: Complex I and II
12.8K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
12.8K
The Proteasome
827
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
827
Export of Misfolded Proteins out of the ER
3.6K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.6K
Delivery Pathways to the Lysosome
6.2K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.2K


