活性蛋白A通过诱导细胞外囊泡的产生来调节B级急性淋巴细胞白血病细胞通信和生存
Eugenia Licari1, Giulia Cricrì1,2, Mario Mauri3
1Tettamanti Center, Fondazione IRCCS San Gerardo dei Tintori, Via Pergolesi, 20900, Monza, Italy.
Scientific reports
|July 11, 2024
概括
活性蛋白A增强了B细胞急性淋巴细胞白血病 (B-ALL) 细胞中的细胞外囊 (EV) 释放和吸收. 这种EV介导的交叉声会在压力条件下促进白血病细胞的存活.
科学领域:
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
- 免疫学 免疫学 免疫学
背景情况:
- 细胞外囊泡 (EVs) 调解细胞间通信,影响瘤生存和抗压力.
- 在B细胞急性淋巴细胞白血病 (B-ALL) 中EVs的作用需要进一步调查.
- 已经证明,ActivinA会影响B-ALL细胞中的水平和动素聚合,这些过程与EV动态有关.
研究的目的:
- 调查电动汽车在B-ALL中的作用.
- 为了确定ActivinA对B-ALL中EV生产和功能的影响.
- 评估EV介导交叉声对压力下的B-ALL细胞存活率的影响.
主要方法:
- 用ActivinA治疗B-ALL细胞.
- 电动汽车的隔离和特征.
- 涉及B-ALL细胞和EVs的共同培养实验.
- 在培养引起的压力下评估细胞活力.
主要成果:
- 活性蛋白A治疗增加了白血病细胞释放的EV的数量.
- B-ALL细胞从其他B-ALL细胞中积极内化了EVs.
- 在压力下,EV共同培养促进了剂量依赖的B-ALL细胞存活率.
- 与单独使用ActivinA相比,ActivinA刺激或使用ActivinA诱导的EVs治疗更有效地提高了B-ALL细胞存活率.
结论:
- 在B-ALL中,ActivinA显著增强了EV介导的交叉通话.
- 这种增强的交叉连接有助于改善在压力条件下的白血病细胞存活率.
- 在B-ALL中,ActivinA可能会影响EV的产生和载荷 (例如,微RNA).
相关概念视频
The Intrinsic Apoptotic Pathway
6.5K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.5K
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
The Extrinsic Apoptotic Pathway
6.3K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.3K
B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
Canonical Wnt Signaling Pathway
8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K


