乳酸盐通过H3K18乳化而不是H3K27乙化来调节异位基因组激活,而不是通过H3K27乳化
Yanhua Zhao1, Meiting Zhang1, Xingwei Huang1
1Department of Histology and Embryology, Harbin Medical University, Harbin, 150081, China.
Cellular and molecular life sciences : CMLS
|July 11, 2024
概括
乳酸对小鼠早期胚胎发育至关重要,防止细胞循环停止. 它的缺失会通过改变基因激素乳化 (H3K18la) 来损害关键的发育过渡,这是对基因激活至关重要的表观遗传修饰.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展生物学 发展生物学
- 生物化学 生物化学
背景情况:
- 乳酸在早期胚胎发育中的作用尚未完全理解.
- 基因组乳基化是一种新兴的表观遗传修饰,它对各种细胞过程有影响.
- 以前的研究表明,乳酸积累可能会影响基因素乙化.
研究的目的:
- 研究乳酸对小鼠早期胚胎发育的精确影响.
- 探索质母乳化,特别是H3K18la在调解乳酸的作用中的作用.
- 为了确定乳酸是否会影响母细胞转化 (MZT) 和胚胎基因激活 (ZGA).
主要方法:
- 在含有和不含乳酸的介质中培养小鼠胚胎.
- 使用RNA测序 (RNA-seq) 来分析基因表达变化.
- 执行Cut&Tag测定以评估基因素乳化 (H3K18la) 和乙化 (H3K27ac) 修改.
主要成果:
- 缺少乳酸导致胚胎在G2阶段晚期发生显著的2细胞停止.
- 乳酸缺乏会影响母体转化为胚胎的转变 (MZT) 和胚胎基因激活 (ZGA).
- 在乳酸缺乏下观察到H3K18la修饰的显著减少,与改变的基因表达相关,而H3K27ac显示的相关性很小.
结论:
- 乳酸对于成功的早期小鼠胚胎发育至关重要,防止细胞循环停止.
- 乳酸盐主要通过表观遗传修饰H3K18la影响胚胎发育,影响MZT和ZGA.
- 基因素乳化 (H3K18la) 是乳酸在早期胚胎发生过程中调节基因表达的关键机制.
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