流的剪切力通过影响CHOP和GRP78蛋白质来破坏 carotid斑块的稳定
Dong Chen1, Liding Wang2, Tao Jiang3
1Dalian University of Technology, China; Dalian University of Technology Affiliated Central Hospital, China.
概括
低血流剪切力增加GRP78和CHOP的表达,可能会破坏心血管斑块的稳定,增加中风风险. 这突显了动脉样硬化斑块不稳定的关键因素.
科学领域:
- 心血管研究研究心血管研究
- 生物医学工程 生物医学工程
- 分子生物学分子生物学
背景情况:
- 缺血性中风通常是由不稳定的喉动脉样硬化斑块引起的,其中18-25%与斑块破裂有关.
- 斑块稳定性对于患者的预后至关重要,需要准确的方法来评估它.
- 以前的研究将CHOP (C/EBP同源蛋白) 和GRP78 (葡萄糖调节蛋白78) 的表达与斑块稳定性联系起来.
研究的目的:
- 为了研究血液流动剪切力对内皮细胞GRP78和CHOP表达的影响.
- 实证地研究剪切力如何影响人动脉动脉硬化斑块的稳定性.
- 探索血液动力学因素在动脉斑不稳定中的作用.
主要方法:
- 人的静脉内皮细胞受到低,中,高切削力 (4,8,12天/厘米2) 的影响.
- 使用西部斑块和RT-qPCR量化了GRP78和CHOP蛋白和mRNA水平.
- 来自60名患者的斑块被分类为稳定或不稳定,在动脉分叉处测量了剪切力.
主要成果:
- 与中高剪切相比,低剪切力显著提高了内皮细胞中的GRP78和CHOP表达.
- 不稳定的动脉斑块在分叉处表现出明显较低的剪切力,而不是稳定的斑块.
- 与稳定的相比,不稳定的斑块中的GRP78和CHOP表达水平显著更高.
结论:
- 血流剪切力在血管内皮细胞中差异调节GRP78和CHOP的表达.
- 降低的切削力和较低的流体流速与增加的GRP78和CHOP表达相关.
- 升高的GRP78和CHOP可能会诱导内分泌网膜应激,导致状腺斑块不稳定和中风风险.
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