相关实验视频

Updated: Jun 21, 2025

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

阿尔法-黑色素细胞刺激激素有助于对脂多聚糖的抗炎反应

R P Reynolds¹, R R Fan¹, A Tinajero¹

¹Department of Internal Medicine, Center for Hypothalamic Research, Dallas, TX, USA.

Molecular metabolism

|July 11, 2024

概括

内源性α-黑色素细胞刺激激素 (α-MSH) 对于调节对细菌脂聚糖 (LPS) 的免疫反应至关重要. 缺乏α-MSH的小鼠表现出过度的炎症和代谢功能障碍,突出了它在控制炎症状况方面的作用.

科学领域:

免疫学和新陈代谢
神经内分泌学神经内分泌学

背景情况:

亲opiomelanocortin (POMC) 分裂产物,包括α-MSH,在大脑和垂体中释放.
α-MSH调节能量平衡并具有抗炎性质,但其在生理抗炎反应中的作用尚不清楚.
开发了一种新的小鼠模型 (Pomc^tm1/tm1) 来研究内源性α-MSH的功能.

研究的目的:

为了确定是否需要内源性α-MSH来调节急性炎症期间的免疫反应.
为了比较具有和没有α-MSH生产的小鼠之间对脂聚糖 (LPS) 的炎症和代谢反应.

主要方法:

雄性Pomc^tm1/tm1和野生型Pomc^wt/wt小鼠被注射了盐水或低剂量的LPS.
监测免疫和代谢参数,包括核心体温,循环细胞因子,皮质和α-MSH水平.
测量包括体重,食物摄入,活动,呼吸,以及通过质谱测量测量下丘脑/垂体α-MSH度.

主要成果:

与对照组相比,Pomc^tm1/tm1小鼠对LPS的免疫反应过度.
缺乏α-MSH的小鼠在LPS后经历了更严重的低温,减少了氧气消耗和代谢反应受损.
在Pomc^tm1/tm1小鼠中观察到增高的促炎细胞因子,而野生型小鼠在LPS后显示出循环α-MSH的增加.

结论:

内源性α-MSH在调节对LPS的炎症性免疫反应方面发挥着重要作用.
准黑色皮质素系统可能为治疗败血症和炎症性疾病提供治疗策略.
这些发现强调了由POMC系统调节的新陈代谢和免疫之间的联系.

关键词:

在LPS中使用LPS.鼠标模型模型在POMC中,POMC是POMC.热生成是一种热生成.α-MSHH 的情况.

更多相关视频

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Published on: May 2, 2018

相关概念视频

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Inflammation

Inflammation