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LncRNA SNHG5/IGF2BP1/Occludin轴调节 Nd2O3诱导的血液和丸屏障的破坏
Shurui Wang1, Yupeng Ma1, Wenjie Li1
1School of Public Health, Baotou Medical College, Baotou, 014030, Inner Mongolia, PR China.
Environmental pollution (Barking, Essex : 1987)
|July 11, 2024
概括
氧化物暴露会通过破坏血液丸屏障损害男性生育能力. 这通过 lncRNA SNHG5 发生,这减少了 IGF2BP1,影响了 Occludin 和屏障完整性.
科学领域:
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
背景情况:
- 新氧化物 (Nd2O3) 是一种稀土元素,可导致器官损伤.
- 由于Nd2O3对男性生殖系统的危害,尚不清楚.
- 像SNHG5这样的长非编码RNA与疾病进展有关.
研究的目的:
- 调查 lncRNA SNHG5 在 Nd2O3 诱导的男性生殖毒性的作用.
- 阐明 Nd2O3 暴露与男性不孕症相关的分子机制.
- 确定与Nd2O3相关的生殖损伤的潜在治疗点.
主要方法:
- 鼠被暴露在Nd2O3中,以评估丸的影响.
- 测量了SNHG5和IGF2BP1的表达水平.
- 在体内和体外实验中检查了蛋白质-蛋白质相互作用和mRNA稳定性.
- 评估了血液测试屏障 (BTB) 的透性和紧结蛋白 (Occludin) 的完整性.
主要成果:
- Nd2O3暴露显著增加了小鼠丸中的SNHG5表达.
- SNHG5直接与IGF2BP1蛋白水平结合并降低.
- 这种相互作用通过影响Occludin mRNA稳定性来破坏BTB细胞骨架.
- 在暴露于Nd2O3后,观察到BTB的透性增加.
结论:
- SNHG5/IGF2BP1/Occludin轴是Nd2O3诱导的男性生殖器损伤的一个关键机制.
- 了解这种途径为治疗与Nd2O3相关的男性不孕症提供了洞察力.
- SNHG5和IGF2BP1是暴露于有毒物质下的BTB完整性的关键调节者.
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