Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Cell Migration01:19

Cell Migration

4.8K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
4.8K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.6K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.6K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.2K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.2K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

2.3K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.3K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Engineering with EP2/EP4 knockout and IL-15 transpresentation renders stem cell-derived NK cells self-persistent and resistant to PGE2 inhibition.

Frontiers in immunology·2026
Same author

IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species.

PLoS biology·2026
Same author

Dose-dependent effects and mechanisms of exercise-like stimulation on cardiac injury and contractile function: outcomes of the MICRO-ATHLETE study.

Basic research in cardiology·2026
Same author

The Landscape of Pediatric Differentiated Thyroid Carcinoma and Predictors of Invasive Disease: A CATC Study.

The Journal of clinical endocrinology and metabolism·2026
Same author

Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine candidate in mice.

NPJ vaccines·2026
Same author

<i>KEAP1</i> mutations activate the NRF2 pathway to drive cell growth and migration, and attenuate drug response in thyroid cancer.

Frontiers in oncology·2026

相关实验视频

Updated: Jun 21, 2025

Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

11.9K

在对齐的光纤网络上的树突细胞力迁移合.

Christian Hernandez-Padilla1, Ben Joosten2, Aime Franco3

  • 1Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia.

Biophysical journal
|July 12, 2024
PubMed
概括

不成熟的树突细胞 (iDCs) 在3D纤维环境中表现出独特的迁移行为,在持久和缓慢状态之间过渡. 它们利用丝状突出来导航和对纤维施加力量,独立于对齐,揭示了对体内免疫细胞运动的洞察力.

更多相关视频

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
11:28

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Published on: March 25, 2016

10.7K
Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration
06:42

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

Published on: May 24, 2024

1.4K

相关实验视频

Last Updated: Jun 21, 2025

Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

11.9K
Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
11:28

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Published on: March 25, 2016

10.7K
Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration
06:42

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

Published on: May 24, 2024

1.4K

科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 树突细胞 (DCs) 是关键的抗原呈现细胞,启动了适应性免疫.
  • 通过连接组织的DC迁移对于免疫监测至关重要.
  • 在3D纤维环境中对DC运动的理解有限.

研究的目的:

  • 研究未成熟的树突细胞 (iDC) 在模仿细胞外基质 (ECM) 的3D纤维网络中的迁移.
  • 描述iDC的迁徙行为,突出动态和对地形线索的反应.
  • 探索纤维直径和对齐对iDC运动性的影响.

主要方法:

  • 利用悬浮光纤网络来模拟3D ECM环境.
  • 使用活细胞成像观察和分析iDC迁移模式.
  • 研究了纤维拓和炎症剂 (PGE2) 的反应中的细胞突起和行为组织.
  • 量化的iDC力合和迁移动态.

主要成果:

  • iDC显示了迁移周期,在3D光纤网络中在持久和缓慢状态之间转移.
  • 一个iDCs的子集形成了前向突出,在PGE2处理后反转.
  • iDCs表现出强力合和侧向附着在纤维上,使纤维对齐无关迁移.
  • 增加的纤维直径 (200-500 nm) 并没有改变迁移,但诱导了更密集的活性束.

结论:

  • 在3D纤维环境中iDC迁移的特点是动态循环和独特的力合机制.
  • 地形线索,包括纤维对齐和直径,影响iDC突起形成和活动组织.
  • 这些发现为iDC如何在复杂的体内组织环境中导航提供了新的见解.