从珍珠小米中收集和分析RNA测序数据的数据
Kota Kambara1, Shashi Kumar Gupta2, Tetsuo Takano1
1Asian Research Center for Bioresource and Environmental Sciences (ARC-BRES), Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Midori-cho, Nishi-tokyo-shi, Tokyo 188-0002, Japan.
Data in brief
|July 12, 2024
概括
珍珠小米是一种弹性C4作物,使用来自565个样本的RNA测序数据进行了分析. 这项研究提供了有价值的基因表达数据,以了解其耐压力机制.
科学领域:
- 农业科学 农业科学
- 植物生物学 植物生物学
- 基因组学就是基因组学.
背景情况:
- 珍珠小米 (Pennisetum glaucum/Cenchrus americanus) 是一种重要的C4谷物作物.
- 它对干旱,高温和营养不良的土壤表现出了显著的耐受性.
- 了解其遗传机制对于作物改进至关重要.
研究的目的:
- 编制和标准化珍珠小米的RNA测序数据.
- 为珍珠小米研究创建一个全面的基因表达资源.
- 促进对珍珠小米发育和应激耐受性的研究.
主要方法:
- 收集的RNA测序 (RNA-Seq) 来自25个NCBI生物项目中的565个珍珠小米样本.
- 映射RNA-Seq可以读取珍珠小米参考基因组.
- 量化基因表达水平作为每百万读数和转录 (TPM).
主要成果:
- 创建了565个珍珠小米样本的基因表达特征统一数据集.
- 关联表达式数据与样本属性和项目信息.
- 在figshare存储库中存储了全面的数据集,以便公众访问.
结论:
- 该研究为珍珠小米研究提供了有价值的,可访问的资源.
- 这些数据将有助于剖析珍珠小米抗压能力的遗传基础.
- 促进未来对作物改良和适应策略的研究.
相关概念视频
RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...


