通过普遍保留的ATPase控制化学伴侣
Hong Jiang1,2,3, Martin Milanov1,2,3, Gabriela Jüngert1
1Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.
iScience
|July 12, 2024
概括
删除YchF通过增加RpoS和多酸盐来增强细菌的抗压能力. 这表明细菌和真核生物中耐压力的统一机制.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- YchF/Ola1 ATPases对于调节原核生物和真核生物的应激反应途径至关重要.
- 删除YchF/Ola1会增加对环境压力因素的抵抗力,例如反应性氧物种.
- YchF/Ola1的升级与人类瘤发生有关.
研究的目的:
- 研究YchF缺失增强大肠杆菌抗压力的机制.
- 探索RpoS和多酸盐在YchF介导的应激反应中的作用.
- 确定细调聚酸盐水平对于细菌生存的必要性.
主要方法:
- 在大肠杆菌中进行基因删除研究 (例如,ICHF删除).
- 对替代性西格玛因子RpoS水平的分析.
- 多酸盐和多酸盐激酶活性的量化.
- 通过双重基因删除 (*ychF*和外聚酸酶) 调查合成致死性.
主要成果:
- 缺少YchF通过转录独立途径刺激RpoS合成.
- 升高的RpoS增强了主要应激反应基因的转录.
- 删除 *ychF* 增加了聚酸盐激酶,促进了聚酸盐的产生.
- 同时删除ychF和exopolyphosphatase导致大肠杆菌中的合成致死性.
结论:
- 通过YchF/Ola1删除,通过RpoS和多酸盐增强了细菌的抗压能力.
- 聚酸盐的生产对于细菌的生存至关重要,需要微调.
- 这项研究为YchF/Ola1在跨物种抗压能力中的作用提供了一个统一的概念.
更多相关视频
相关概念视频
Molecular Chaperones and Protein Folding
17.9K
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
The...
17.9K
Energy to Drive Translocation
2.1K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
Generally, polypeptides are unfolded by two distinct...
2.1K
Mechanical Protein Functions
4.9K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
4.9K
Allosteric Proteins-ATCase
5.7K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.7K
ATP Synthase: Structure
12.2K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
12.2K
ATP Synthase: Mechanism
14.4K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
14.4K


