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在使用黄单核酸 (FMN) 的HOPE期间对移植的实时评估 - 一项临床前研究

Richard X Sousa Da Silva¹, Tom Darius^2,3, Leandro Mancina¹

¹Swiss HPB and Transplantation Center, Department of Surgery and Transplantation, University Hospital Zurich, Zurich, Switzerland.

Frontiers in transplantation

|July 12, 2024

概括

在低温氧化输液 (HOPE) 期间的弗拉单核酸 (FMN) 水平可以评估移植损伤. 这种方法提供了接受高风险脏的客观标准,可能弥合了供体可用性和患者需求之间的差距.

科学领域:

移植研究移植研究
器官保存策略器官保存策略
发现生物标志物的发现.

背景情况:

脏供体移植与移植等候名单之间的差异越来越大,需要使用高风险器官.
目前对机器透脏的评估缺乏客观标准,特别是对于低温氧化 perfusion (HOPE).
线粒体衍生的黄素单核酸 (FMN) 已经显示出对肝移植功能的预测价值.

研究的目的:

评估在HOPE期间使用输液性FMN测量来评估移植损伤的可行性.
为了将FMN释放与供体温暖缺血时间 (WIT) 和损伤标志物相关联.
探索FMN作为接受边缘移植的客观工具.

主要方法:

使用猪 perfusion 模型来模拟不同的热缺血时间 (0, 30, 60 分钟).
脏经历了静态冷藏,随后进行了2小时的终性缺血期望与实时FMN测量.
FMN水平与损伤相关的分子模式 (DAMPs) 和腺三酸盐 (ATP) 生产能力相关.

主要成果:

perfusate FMN测量在 perfused 脏中是可行的.
在60分钟WIT的脏中,与30分钟和对照组相比,观察到明显更高的FMN释放.
FMN释放与DAMP (8-OHdG,HMGB1) 相相关,与ATP补充能力相反.

结论:

在HOPE期间实时测量FMN是评估移植损伤的可行和客观方法.
FMN量化与先前存在的损伤和捐赠者的WIT相关.
这种方法可以帮助客观地选择高风险的移植,可能增加器官利用率并减少移植后的功能障碍.

关键词:

在循环死亡后的捐赠.扩展标准捐赠者捐赠者弗拉单核酸的一种单核酸.低温氧化的机器 perfusion perfusion 是一个低温的氧化机器.腎臟移植腎臟移植器官康复器官康复实时评估实时评估静态冷藏库的冷藏库是什么

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