七的进化起源和祖先的特征
Samed Delic1, Brent Shuman2, Shoken Lee3
1Department of Biology, Duke University, Durham, NC, United States.
Frontiers in cell and developmental biology
|July 12, 2024
概括
七素,关键的细胞骨蛋白质,起源于真核生物进化的早期. 它们的古老结构揭示了膜结合是祖先的特征,进化使它们的相互作用多样化.
科学领域:
- 进化生物学是进化的生物学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 七素是细胞分裂和膜动力学中的重要细胞骨蛋白质.
- 它们的进化起源和在真核生物中祖先的功能尚未得到充分理解.
- 七面体在各种真核细胞系中得到保存,这表明其起源很古老.
研究的目的:
- 为了澄清细胞系关系和eukaryotes跨越septins的进化历史.
- 为了研究七的祖先结构特征和功能.
- 了解隔膜相互作用和膜关联的多样化.
主要方法:
- 来自各种真核生物群体的254个septin序列的家族遗传学分析.
- 祖先序列重建和基于AlphaFold的结构预测.
- 保存的残留物和结构图案的识别,包括阿尔金林指和两螺旋.
主要成果:
- 观察到非opisthokont和opisthokont隔膜之间的明显分叉.
- 确定了三个主要的非opisthokont septin组 (6,7和8),在光植物和状动物中具有特定的血统.
- 在GTPase域中保留了一根阿基宁指,表明祖先的同质化,其损失可能会导致向异质聚合和膜结合的多样化.
结论:
- 七是一种古老的蛋白质,具有膜结合的祖先特征.
- 进化损失的阿基尼因手指和α0螺旋的收益促进了隔膜相互作用的多样化.
- 这项研究提供了一个框架,以了解膜进化和真核细胞的功能多样化.
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