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单细胞RNA测序显示,NRF2调节了腹腔大动脉动脉瘤中的血管光滑肌细胞表型切换

Xiaoyong Xiao¹, Chenglin Li¹, Xiaojia Huang²

¹Department of Emergency Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

|July 12, 2024

概括

核因子E2相关因子2 (NRF2) 损失通过促进血管光滑肌细胞 (VSMC) 切换而加剧腹腔大动脉动脉瘤 (AAA). NRF2通过microRNA-145保持VSMC收缩表型,影响AAA的发展.

科学领域:

血管生物学血管生物学
分子医学是分子医学.
遗传学遗传学是一个

背景情况:

腹腔大动脉瘤 (AAA) 涉及血管壁破坏和血管光滑肌细胞 (VSMC) 现型切换.
了解VSMC监管因素对于AAA治疗策略至关重要.

研究的目的:

研究核因子E2相关因子2 (NRF2) 在调节VSMC表型和AAA发展中的作用.
阐明NRF2对AAA病原体产生影响的分子机制.

主要方法:

在小鼠AAA模型中进行单细胞RNA测序分析.
产生特定于VSMC的NRF2淘汰小鼠.
ангиотензинII诱导的AAA模型.
全基因组转录组分析和功能丧失/获取实验.

主要成果:

在VSMC中NRF2缺陷加剧了AAA形成.
低调NRF2促进了VSMC表型切换和炎症.
通过促进microRNA-145 (miR-145) 的表达,NRF2提高了VSMC收缩型表型基因的调节.

结论:

NRF2是一种新型调节剂,维持VSMC收缩性表型.
NRF2通过一种依赖于miR-145的机制影响AAA的形成.

关键词:

腹部大动脉动脉瘤腹部大动脉动脉瘤与核因子E2相关的核因子2因子.现型切换的切换方式血管光滑肌肉细胞细胞

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