从氧化预处理的小麦中生产蜂酸盐,由工程Neurospora crassa HL10进行
Jiajie Wang1, Takao Kasuga2,3, Zhiliang Fan4
1Department of Biological and Agricultural Engineering, University of California , Davis, One Shields Avenue, Davis, CA, 95616, USA.
Bioprocess and biosystems engineering
|July 12, 2024
概括
这项研究表明,使用Neurospora crassa HL10.10从小麦中有效地生产蜂酸盐. 该过程利用天然素作为中间体,并且在没有外部酶或中间体的情况下获得高产量.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 微生物生产 微生物生产
背景情况:
- 蜂酸的生产通常需要纯基质和酶.
- 基纤维素生物质提供了一个可持续但具有挑战性的原料.
- 神经毛虫 (Neurospora crassa) 具有生物转化代谢能力.
研究的目的:
- 通过使用Neurospora crassa HL10.10 来研究从红细胞纤维素基质中产生蜂酸盐的生产.
- 通过解决乳糖生产的局限性来优化生物转化过程.
- 评估使用预处理的小麦作为低成本原料的效率.
主要方法:
- 使用 NaOH 预处理的小麦作为纤维素基质.
- 雇佣了神经虫 (Neurospora crassa) HL10用于细胞酸盐的生产.
- 应用循环赫西米德来诱导乳糖酶的产生.
- 量化纤维酸产量和纤维素转化率.
主要成果:
- 预处理的小麦中的红素充当了天然的氧化还原媒介.
- 低内源性乳糖生产限制了初始转换率.
- 循环赫西米德诱导显著增强了乳糖酶的产生和纤维素酸盐产量 (57 mM).
- 在6天内实现了92%的纤维素转化为纤维氨酸.
结论:
- 神经草草 HL10 可以有效地从预处理的小麦中生产蜂酸盐.
- 循环赫西米德诱导的乳酶生成是加速生物转化过程的关键.
- 这种方法提供了一种成本效益高且可持续的途径,可以在没有外部酶或介质补充的情况下生产蜂酸.
更多相关视频
相关概念视频
Microbial Fermentation
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...


