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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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相关实验视频

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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
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使用MODplus解密PTM识别:最佳实践和陷

Seungjin Na1, Eunok Paek2,3,4

  • 1Digital Omics Research Center, Korea Basic Science Institute, Cheongju, South Korea.

Methods in molecular biology (Clifton, N.J.)
|July 12, 2024
PubMed
概括

这项研究增强了使用双重质谱法 (MS/MS) 的翻译后修饰 (PTM) 识别. 它引入了MODplus软件,用于更快,更广泛的PTM分析,解决复杂的生物样本中的计算挑战.

关键词:
计算型蛋白质组学是一种计算型蛋白质组学.在MS/MS/MS上使用.打开搜索 打开搜索在PTM中,PTM是PTM.软件 软件 软件 软件 软件协奏式质谱测量 质谱测量后翻译修改后的修改.

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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
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科学领域:

  • 生物化学 生物化学
  • 蛋白质组学是指蛋白质组学.
  • 计算生物学 计算生物学

背景情况:

  • 翻译后修饰 (PTM) 对于调节生物过程至关重要.
  • 双重质谱 (MS/MS) 是识别PTM的一个关键技术.
  • 目前的方法面临的挑战是速度和可检测的PTM的范围.

研究的目的:

  • 审查使用MS/MS的PTM检测的最新进展.
  • 在同时进行多个PTM搜索时应对计算挑战.
  • 为广泛的PTM识别提供自动化程序.

主要方法:

  • 探索用于PTM发现的先进MS/MS技术.
  • 讨论复杂的PTM搜索的计算策略.
  • 介绍和演示用于PTM分析的MODplus软件.

主要成果:

  • 最近的进展扩大了PTM识别的范围和速度.
  • MODplus提供了一个自动化工作流程,用于全面的PTM分析.
  • 一个案例研究说明了MODplus程序的实际应用和结果.

结论:

  • 提出的方法和MODplus软件提高了PTM识别的效率和广度.
  • 这项工作有助于通过PTM来更深入地了解生物调节.
  • 自动化PTM分析对于推动蛋白质组研究至关重要.