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相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

740
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
740
Mass Spectrometers01:16

Mass Spectrometers

5.3K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.3K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.0K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.0K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

4.1K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
4.1K

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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
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让MS Omics数据 ML-Ready:SpeCollate协议的使用

Muhammad Usman Tariq1, Samuel Ebert1, Fahad Saeed2

  • 1Knight Foundation School of Computing and Information Sciences (KFSCIS), Florida International University (FIU), Miami, FL, USA.

Methods in molecular biology (Clifton, N.J.)
|July 12, 2024
PubMed
概括
此摘要是机器生成的。

本指南详细介绍了用于机器学习 (ML) 应用的质谱 (MS) 数据的转换. 它介绍了SpeCollate深度学习模型用于类推断,增强了蛋白质组学数据分析.

关键词:
数据预处理数据的预处理.数据库搜索搜索数据库搜索深度学习是一种深度学习.机器学习是机器学习.酸标识 酸标识协奏式质谱测量 质谱测量 协奏式质谱测量蛋白质组学是指蛋白质组学.

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 计算生物学 计算生物学
  • 数据科学数据科学数据科学

背景情况:

  • 质谱 (MS) 产生复杂,大量的数据,对蛋白质组学分析构成挑战.
  • 现有的鉴定方法通常依赖于简化的光谱模拟和启发性评分.

研究的目的:

  • 为机器学习 (ML) 模型训练和推理转换MS数据提供全面指南.
  • 介绍和演示深度学习模型SpeCollate的应用,用于蛋白质组学中的谱匹配.

主要方法:

  • 用于MS数据的数据预处理和转换技术.
  • 开发和应用SpeCollate深度学习模型,用于联合光谱和嵌入.
  • 从多发性硬化病数据中推断的逐步指南.

主要成果:

  • SpeCollate有效地克服了传统方法的局限性,通过生成光谱和的联合嵌入.
  • 展示了一个用户友好的命令行界面用于基数据库搜索.
  • 突出了ML在推进MS数据分析方面的潜力.

结论:

  • 该章授权研究人员利用ML获得基于MS的OMICS的新见解.
  • SpeCollate提供了一种有效和创新的鉴定方法.
  • 有效的数据转换对于蛋白质组学中的ML应用成功至关重要.