Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows.

PNAS nexus·2026
Same author

Scalable Flow Reactors for Stable Biofilm Formation and Continuous Whole-Cell Catalysis.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Carrier-Free Enzyme Immobilization for High-Density Catalytic Architectures in Flow Biocatalysis.

JACS Au·2026
Same author

Low-Cost Temperature Sensing Reveals Thermal Signatures of Microbial Activity in Winogradsky Columns.

Sensors (Basel, Switzerland)·2025
Same author

Diagnosis of Coronary Artery Aneurysm in a Caucasian Population Cohort: Evaluating the Agreement Between Japanese Criteria and Different Z Score Formulas.

Journal of clinical medicine·2025
Same author

Clustering of Membrane Receptors: Insights from DNA Origami-Based Approaches.

Small (Weinheim an der Bergstrasse, Germany)·2025

相关实验视频

Updated: May 7, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

14.6K

基于RCA的DNA水凝的定量表征 - - 向理性材料设计的方向

Svenja A Moench1, Phillip Lemke1, Julia Weisser1

  • 1Institute for Biological Interfaces (IBG-1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1, Eggenstein-Leopoldshafen, 76344, Germany.

Chemistry (Weinheim an der Bergstrasse, Germany)
|July 12, 2024
PubMed
概括

通过滚动圆放大 (RCA) 优化DNA水凝合成对于生物医学用途至关重要. 模板度显著影响DNA片的产生和水凝的特性,指导未来的材料设计.

关键词:
它们是DNA DNA DNA DNA.这是一种DNA水凝.盖尔斯 (Gels) 是一种凝.滚动圈放大器 滚动圈放大器粘度 粘度 粘度 是一种

更多相关视频

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.2K
Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

6.7K

相关实验视频

Last Updated: May 7, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

14.6K
The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.2K
Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

6.7K

科学领域:

  • 生物材料科学 生物材料科学
  • 分子生物学分子生物学
  • 聚合物化学 聚合物化学

背景情况:

  • DNA水凝显示出生物医学应用的潜力.
  • 酶式滚动圆放大 (RCA) 是一种用于DNA水凝合成的方法.
  • 在水凝合成中缺乏RCA的标准化协议.

研究的目的:

  • 研究各种反应参数对RCA效率和DNA水凝特性的影响.
  • 为合理的DNA水凝设计建立结构-属性关系.

主要方法:

  • 对于RCA.不同的模板序列和试剂度.
  • 使用定量PCR (qPCR) 评估RCA合成效率.
  • 通过缩测量评估水凝的机械性能.

主要成果:

  • 循环模板度对RCA生产率的影响最大.
  • 聚合酶和核酸度影响了RCA的效率.
  • 水凝粘度与DNA片度呈指数相关性.
  • 核基序列影响了放大和材料特性,特别是通过二次结构.

结论:

  • 通过了解结构-属性关系,可以实现DNA水凝的理性设计.
  • 将高通量实验与折叠预测相结合,有助于系统的材料开发.
  • 优化的RCA协议对于推进DNA水凝应用至关重要.