亚酸盐通过线粒体功能障碍和ERS反应诱导斑马鱼的肝糖和脂质代谢障碍
Hui Yang1, Kang Ou-Yang1, Ya He1
1College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China.
Aquatic toxicology (Amsterdam, Netherlands)
|July 12, 2024
概括
化物暴露会破坏葡萄糖和脂质代谢,从而伤害水生动物. 这发生在线粒体的氧化应激和内质网膜应激,影响肝功能和整体健康.
科学领域:
- 环境毒理学环境毒理学
- 水生动物生理学 水生动物生理学
- 代谢生物化学 代谢生物化学
背景情况:
- 酸盐是一种有毒的环境污染物,影响水生生物.
- 了解酸盐对葡萄糖和脂质代谢的影响对于水生动物健康至关重要.
研究的目的:
- 为了研究酸盐暴露对斑马鱼葡萄糖和脂质代谢的体内影响.
- 阐明酸盐诱导的代谢干扰背后的分子机制.
主要方法:
- 斑马鱼暴露于不同度的亚酸盐 (0,0.2,2,20 mg/L).
- 评估了线粒体氧化应激标志物 (MDA,ROS,MMP,ATP) 和内质网膜应激标志物 (ERs).
- 分析的基因表达与葡萄糖代谢 (HIF-1α途径,糖解,葡萄糖生成) 和脂质代谢 (脂解,脂质合成) 相关.
- 在体外研究中使用了Mito-TEMPO和TUDCA来抵消酸盐的作用.
主要成果:
- 酸盐暴露诱导了斑马鱼肝脏和ZFL细胞中的线粒体氧化应激和ERs.
- 通过HIF-1α通路激活和改变关键基因表达来破坏葡萄糖代谢.
- 通过增加脂解和降低脂合成基因表达,降低脂代谢.
- 试验室治疗减轻了酸盐诱导的氧化应激,ERs和代谢功能障碍.
结论:
- 酸盐暴露会破坏斑马鱼的肝脏葡萄糖和脂质代谢.
- 线粒体功能障碍和ER是酸盐诱导肝毒性的关键机制.
- 这些发现有助于更好地了解酸盐对水生动物的毒性影响.
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