相关实验视频

Updated: Aug 17, 2026

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

通过双重重组合和双重光追踪细胞膜和细胞核的同步谱系

Xueying Yang¹, Shun He², Xufeng Li¹

¹School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310024, China.

Journal of genetics and genomics = Yi chuan xue bao

|July 12, 2024

概括

这项研究引入了使用Cre和Dre重组的新型遗传谱系追踪系统,用于精确的细胞命运研究. 这些系统清楚地可视化细胞膜和细胞核,提高发育和疾病研究的准确性.

科学领域:

细胞生物学细胞生物学
遗传学遗传学是一个
发展生物学发展生物学

背景情况:

遗传谱系追踪对于了解细胞命运至关重要,但在细胞边界可视化和特异性方面面临挑战.
现有的Cre-loxP系统在准确追踪不同细胞种群方面存在局限性.

研究的目的:

开发和验证新的遗传报告系统,以加强细胞谱系的追踪.
改进细胞边界和细胞内部件的可视化,以精确地绘制命运.

主要方法:

使用了基于Cre,Dre的三个报告系统,以及Dre+Cre联合调节的重组.
嵌入了tdTomato用于细胞膜标签和PhiYFP用于核标签.
在心肌细胞和肝细胞中证明有效性,包括静脉内和3D成像.

主要成果:

成功标记了有明显膜和核标记的心肌细胞和肝细胞.
展示了动态细胞膜可视化能力.
在与ProTracer系统相结合时,在追踪肝细胞增殖方面获得了更高的精度.

结论:

开发的双重重组合酶系统为谱系追踪提供了卓越的细胞边界和核可视化.
这些系统显著提高了细胞命运研究在发育,平衡和疾病环境中的精度.

关键词:

细胞膜和细胞核进行标记.克雷洛克斯PPP的创作德雷-罗克斯 (Dre-rox) 是一个双重重组的系统是重组的系统.谱系追踪谱系追踪谱系追踪这是一个ProTracer系统.

更多相关视频

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

相关概念视频

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...