评估ATP导离子通道P2X7受体功能在巨细胞和微质细胞中的技术
Raíssa Leite-Aguiar1, Victória Gabriela Bello-Santos2, Newton Gonçalves Castro2
1Laboratório de Imunofisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Journal of immunological methods
|July 12, 2024
概括
居民巨细胞和微质细胞使用P2X7受体检测危险信号,如腺三酸盐 (ATP). 本综述详细介绍了研究免疫细胞中P2X7受体功能的方法,以获得治疗见解.
科学领域:
- 免疫学 免疫学 免疫学
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
背景情况:
- 居民巨细胞和微质细胞是关键的先天性免疫细胞,维持组织平衡.
- 细胞外腺三酸盐 (ATP) 作为危险信号,激活纯能受体.
- 在巨细胞和微质细胞中高度表达的P2X7受体在炎症和病理状况中起着关键作用.
研究的目的:
- 审查评估P2X7受体功能和分子方面的方法.
- 强调P2X7受体研究在巨细胞和微质细胞中的重要性.
- 为研究人员提供研究P2X7受体信号传导在先天免疫的工具.
主要方法:
- 基因和蛋白质表达分析使用聚合酶链反应 (PCR),西式涂抹,免疫细胞化学和免疫组织化学.
- 通过选择性激动剂/对抗剂的ATP刺激进行功能评估.
- 包括电生理学,细胞内测量,乙基化物吸收和酸测定在内的技术.
主要成果:
- 激活P2X7受体会导致巨细胞和微质细胞的激活,炎症细胞组合,细胞死亡和炎症介质的释放.
- 这些方法可以详细研究P2X7受体介导的信号通路.
- 这些技术对于理解P2X7在神经炎症和其他疾病中的作用至关重要.
结论:
- 对P2X7受体功能和巨细胞和微质细胞中的分子特征的全面了解至关重要.
- P2X7受体信号传递对于健康和疾病中的免疫调节至关重要.
- 讨论的方法为推进P2X7受体研究和确定治疗点提供了有价值的工具.
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相关概念视频
01:27ATP Driven Pumps I: An Overview
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
01:34ATP Driven Pumps II: P-type Pumps
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
01:30ATP Driven Pumps III: V-type Pumps
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...