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化基托纳米颗粒封装尼和:抗微生物/抗生物膜/抗附着/免疫调节多功能剂

Mozhgan Derakhshan-Sefidi¹, Bita Bakhshi², Aliakbar Rasekhi³

¹Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

BMC microbiology

|July 12, 2024

概括

和被封装在化基托纳米颗粒 (N/Se@TCsNPs) 中,显示出强大的抗菌和抗菌膜活性,对抗肠道病原体. 这些纳米粒子还表现出免疫调节作用,减少炎症和病原体粘附.

科学领域:

纳米技术纳米技术
微生物学微生物学
免疫学免疫学免疫学

背景情况:

抗生素耐药性不断增加,需要探索非抗生素化合物的杀菌性.
纳和,封装在硫化酸纳米颗粒 (N/Se@TCsNPs) 中,正在研究它们对常见肠道病原体的潜在作用.

研究的目的:

评估N/Se@TCsNPs的体外抗菌和抗菌膜疗效.
评估N/Se@TCsNPs对Caco-2细胞和细胞因子表达的免疫调节作用.

主要方法:

使用FTIR和SEM等技术合成和描述N/Se@TCsNPs.
苏微稀释和水晶紫色测定抗菌和抗菌膜活性.
对Caco-2细胞的细胞毒性测定和对炎症/抗炎性细胞因子水平 (IL-6,TNFα,IL-10,TGFβ) 和TLR基因表达 (TLR2,TLR4) 的分析.

主要成果:

N/Se@TCsNPs对各种肠道病原体表现出强烈的抗菌活性,有效度在1.5至25毫克/毫升之间.
在Caco-2细胞中显著减少了细菌的粘附和内部化,表明强大的抗菌膜特性.
N/Se@TCsNPs通过降低促炎细胞因子 (IL-6,TNFα) 和增加抗炎细胞因子 (IL-10,TGFβ) 来调节免疫反应,同时降低TLR2和TLR4基因表达的调节.

结论:

N/Se@TCsNPs显示出显著的抗菌,抗菌膜,抗附着和免疫调节效果,对特定的格兰阳性和格兰阴性肠道病原体具有显著的抗菌,抗菌膜,抗附着和免疫调节效果.
进一步的ex-vivo和in-vivo研究是有必要的,以充分评估这些纳米结构N/Se@TCsNPs的治疗潜力.

关键词:

抗生物膜是一种抗生物膜.抗微生物活性的抗菌活性.纳米颗粒纳米颗粒尼辛尼斯尼辛尼斯尼辛尼斯尼辛尼斯是一种.硫化基酸 (thiolated chitosan) 是一种酸的化合物.

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