来自人类尿液衍生干细胞的外体细胞改善IL-1β诱导的脊椎间盘退化
Guang Qian1, Yueming Yu1, Youhai Dong1
1Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, No. 801, Heqing Road, Minhang District, Shanghai, 200240, China.
BMC musculoskeletal disorders
|July 12, 2024
概括
人类尿液衍生干细胞外体可以防止细胞细胞核退化,这是椎间盘退化的关键因素. 这一发现为治疗这种疾病提供了一个有前途的新治疗途径.
科学领域:
- 生物医学科学 生物医学科学
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
背景情况:
- 人类椎间盘退化 (IVDD) 是一个复杂的病理过程.
- 核细胞 (NPC) 退化和内质网膜应激是IVDD的关键驱动因素.
- 没有完全理解IVDD和NPC退化的确切机制.
研究的目的:
- 研究人类尿源干细胞 (USC) 外体在预防椎间盘退化 (IVDD) 的潜力.
- 为了确定USC外体是否可以抵消因特乐金-1β (IL-1β) 诱导的核细胞 (NPC) 退化.
主要方法:
- 建立了一个使用IL-1β刺激NPCs的IVDD模型.
- 利用了西方抹杀,定量实时聚合酶链反应,流细胞计和转录组测序.
- 从人类USCs中提取和特征化了从人类USCs中提取的外体.
主要成果:
- 成功隔离和识别了人类的USC及其外体.
- 证实IL-1β诱导NPC退化,降低活力并改变SOX-9,原II和农草原表达.
- 证明USC外基因组有效地从IL-1β诱导的退化中拯救NPC,恢复关键蛋白质表达水平.
结论:
- 来自USC的外体对IL-1β诱导的NPC退化具有保护作用.
- 这些发现表明,使用USC外体细胞对椎间盘退化 (IVDD) 进行潜在的治疗策略.
更多相关视频
05:46A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.
Published on: February 14, 2021
3.7K
08:13Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice
Published on: April 10, 2019
6.1K
相关概念视频
01:36Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
01:19Mesenchymal Stem Cells
4.7K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.7K