制备微囊和评估其生物控制有效性
Ao Gao1, Lining Zheng1, Shuanglong Wang1
1College of Plant Protection, Jilin Agricultural University, Changchun 130118, PR China.
Journal of bioscience and bioengineering
|July 13, 2024
概括
一种Serratia nematophila L2和Bacillus velezensis W24的组合有效地生物控制了Sclerotinia sclerotiorum,达到88.1%的抑制率. 优化微囊 (GW24Ms) 显示出高生存率和稳定性,减少了88.4%的大豆疾病.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 植物病理学 植物病理学
背景情况:
- 斯克莱罗提尼亚 (Sclerotinia sclerotiorum) 会对大豆作物造成重大破坏.
- 生物防治剂为化学杀菌剂提供了一个可持续的替代品.
研究的目的:
- 评估一种由Serratia nematophila L2和Bacillus velezensis W24组成的微生物药物的疗效,用于对Sclerotinia sclerotiorum进行生物控制.
- 优化培养和微封装条件,以提高疗效和稳定性.
主要方法:
- 优化细菌培养基和条件 (温度,pH,注射剂,旋转速度).
- 微囊制备喷雾干燥参数的优化 (粉度,进气温度,料流量).
- 在大豆植物中评估微囊生存率,细菌数量,储存稳定性和体内疾病控制.
主要成果:
- 在L2与W24的最佳混合比率下,可以实现88.1%的抑制率来抑制S. sclerotiorum的生长.
- 优化的培养条件产生了最大的细菌细胞产量.
- 在优化条件下的微封装导致93.9 ± 0.9%的生存率和6.4 × 10^12 cfu/g的可活细菌数量.
- 在实验中,微囊 (GW24Ms) 显示出良好的存储稳定性,并将大豆疾病降低了88.4%.
结论:
- 塞拉蒂亚线虫L2和Bacillus velezensis W24的结合微生物剂是有效的生物控制的Sclerotinia sclerotiorum.
- 优化的微封装为实际应用提供了稳定且可行的配方.
- 这种微生物剂为控制大豆种植中的斯克勒尼菌病提供了有希望和可持续的解决方案.
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