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咖啡因及其主要代谢物的转移到蛋
Mailson da Silva Teixeira1, Carolina Julia Costa Saraiva1, Bárbara Pereira Dos Santos1
1Laboratório de Toxicologia Veterinária, Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31275-013, Brazil.
Animals : an open access journal from MDPI
|July 13, 2024
概括
咖啡因及其代谢物,神叶素,神胺和帕拉桑丁,转移到蛋中. 这些化合物在蛋白和蛋黄中都被检测到,在蛋白中发现的度更高.
科学领域:
- 食品科学 食品科学 食品科学
- 动物营养 动物营养
- 分析化学 分析化学
背景情况:
- 咖啡因是一种广泛消费的兴奋剂.
- 了解其在蛋等食品中的命运对于食品安全和消费者信息至关重要.
- 以前的研究没有详细说明咖啡因和代谢物转移到蛋中的情况.
研究的目的:
- 为了调查咖啡因及其主要代谢物 (神叶素,神胺,帕拉桑丁) 的存在和水平,在养咖啡因的蛋中.
- 为了比较这些化合物的分布在卵白和蛋黄之间.
- 为了确定在蛋中发现的主要咖啡因代谢物.
主要方法:
- 给产母提供含有0,150,300或450μg/g咖啡因的饮食.
- 卵子在4周,8周和12周收集,用于残留分析.
- 使用光二极管阵列探测器 (HPLC-PDA) 的高性能液态色谱被用于量化咖啡因及其代谢物在蛋白和蛋黄中的含量.
主要成果:
- 咖啡因及其三种主要代谢物被检测到蛋的白和黄.
- 与蛋黄相比,咖啡因和代谢物的度在蛋白中大约是蛋黄的两倍.
- 素是最丰富的代谢物,其次是素;在黄黄中检测到低水平或根本没有素.
结论:
- 咖啡因及其代谢物被转移到蛋中.
- 的饮食咖啡因摄入导致蛋中可检测到的咖啡因及其代谢物水平.
- 分布偏好蛋白而不是蛋黄,而神素是主要发现的代谢物.
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