在肉中对兽医益生菌进行全面的元基因组分析
Ádám Kerek1,2, István László Román1, Ábel Szabó1
1Department of Pharmacology and Toxicology, University of Veterinary Medicine, István utca 2, H-1078 Budapest, Hungary.
Animals : an open access journal from MDPI
|July 13, 2024
概括
肉中的益生菌可能携带抗菌素耐药性基因 (ARG),构成公共卫生风险. 这项研究在家禽益生菌中发现了ARG,但没有证据表明基因通过移动遗传元素转移.
科学领域:
- * 微生物学和家禽科学
- * 基因组学和抗菌素耐药性
背景情况:
- *在肉中使用益生菌来改善肠道健康并减少抗生素的使用.
- * 对益生菌作为抗菌素耐药性基因 (ARG) 的潜在载体和传播者存在担忧,这构成公共卫生风险.
- *在家禽益生菌中研究ARG对于了解潜在的耐药性基因转移至关重要.
研究的目的:
- * 调查用于家禽批准的益生菌中ARG的存在和特征.
- * 评估ARG通过移动遗传元素 (等离子体,菌体) 传递的可能性.
- * 检查用益生菌治疗的肉肠道微生物组和抗体组的变化.
主要方法:
- *使用下一代测序来识别家禽益生菌中的ARG.
- * 在养期间分析了60只肉的肠道微生物组和抗体组.
- *对便样本进行了基因组学分析,以评估微生物组和抗体组的动态.
主要成果:
- *在家禽益生菌中发现了特定的ARG,包括那些对基诺和类抗生素产生耐药性的ARG.
- *检测到具有公共卫生意义的基因 (例如,aadK*,AAC6') -Ii*) 和多个抗药性基因.
- * 随着时间的推移或治疗/饮食组之间没有观察到肠道微生物群或抗体的显著变化;只发现了一种菌体编码的ARG,没有证据表明有等离子体或移动遗传元件的传播.
结论:
- * 禽类益生菌可以携带ARG,突出显示抗菌素耐药性传播的潜在风险.
- *对益生菌分子遗传学的进一步研究对于减轻耐药性基因转移风险至关重要.
- *确保动物养殖中安全使用益生菌需要采取预防ARG传播的策略.
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