作为子子大小的新型候选标记物的MTHFR
Jie Yang1, Zhiyuan Bao1, Jiali Li1
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animals : an open access journal from MDPI
|July 13, 2024
概括
甲基酸酸减少酶 (MTHFR) 基因通过影响颗粒细胞功能和卵巢毛囊发育,影响子 litter 尺寸. 特定的MTHFR促进物多态性与生殖性能有关,具有作为分子标记物的潜力.
科学领域:
- 动物繁殖 动物繁殖
- 分子遗传学 分子遗传学
- 细胞生物学 细胞生物学
背景情况:
- 垃圾大小是动物繁殖的关键经济特征.
- 甲基酸酸减少酶 (MTHFR) 基因在子繁殖中的作用需要进一步研究.
- 了解MTHFR对粒状细胞和卵泡发育的影响,对于提高生殖效率至关重要.
研究的目的:
- 研究MTHFR基因对子颗粒细胞亡和增殖的影响.
- 探索新西兰子中MTHFR基因多态和生殖特征之间的关联.
- 确定MTHFR在卵巢卵泡发育中的调节作用.
主要方法:
- 使用流式细胞计和CCK-8测定来评估颗粒细胞的细胞亡和增殖.
- 使用定量逆转录PCR (qRT-PCR) 和西部斑点 (WB) 分析来检查基因和蛋白质表达.
- 双 luciferase 记者测定和桑格测序被用来识别 MTHFR 促销器区域及其多态.
主要成果:
- MTHFR影响粒状细胞的亡和增殖,并调节关键的毛囊发育基因 (例如TIMP1,CITED1,FSHR,GHR).
- 在MTHFR核心促进体区域中发现了四种单核酸多态 (SNPs).
- 在子中,g.-680C>A多态及其CC基因型与较大的总和活子大小显著相关.
结论:
- MTHFR在调节子卵巢颗粒细胞和卵泡发育方面发挥着重要作用.
- 特定的MTHFR促进体多态性与 litter 尺寸的变化有关.
- 在子中,MTHFR具有作为增强子生殖特征的分子标记物的潜力.
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