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评估封装益生菌 Lactococcus lactis A12 活力使用提拉皮亚的体外消化模型

Marcelo Fernando Valle Vargas¹, María Ximena Quintanilla-Carvajal¹, Luisa Villamil-Diaz¹

¹Grupo de Investigación en Procesos Agroindustriales (GIPA), Doctorado en Biociencias, Facultad de Ingeniería, Universidad de La Sabana, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, 250001 Chía, Cundinamarca, Colombia.

Animals : an open access journal from MDPI

|July 13, 2024

概括

封装的 Lactococcus lactis A12 益生菌在模拟的鱼类胃肠道条件下幸存下来. 这种体外模型有助于在活体试验之前评估水产养殖料中的益生菌生存能力.

科学领域:

水生微生物学水生微生物学
水产养殖中的益生菌
鱼的营养和健康鱼的营养和健康

背景情况:

益生菌对鱼类健康至关重要,但必须在恶劣的胃肠道 (GIT) 中生存.
在体内测试之前,在模拟的GIT条件下评估益生菌生存能力至关重要.
封装可以在运输过程中保护益生菌.

研究的目的:

在模拟的胃和肠道条件下评估封装Lactococcus lactis A12的生存能力.
评估pH值,停留时间和酶度对益生菌生存的影响.
为了比较封装和非封装L. lactis的疗效A12.

主要方法:

采用了模拟鱼类GIT条件的体外消化模型.
一个Box-Behnken设计优化了胃的pH值,居住时间和酶量的评估.
测量了对Streptococcus agalactiae的活力和抗菌活性.

主要成果:

低pH (4.00),延长停留时间 (4小时) 和高酶水平降低了最终细胞数量.
与非封装形式相比,封装的L. lactis A12表现出明显更高的活力 (p < 0.05).
封装中的益生菌也表现出增强的抗菌活性 (p < 0.05) 对S. agalactiae.

结论:

乳球菌乳酸菌A12在模拟鱼类GIT环境中表现出弹性.
封装显著提高了水产养殖中的益生菌生存率和有效性.
开发的体外模型是用于选鱼料中的益生菌的宝贵工具.

关键词:

乳球球菌 (lactococcus lactis) 是一种乳球菌.尼罗河鱼是尼罗河鱼.消化消化消化消化封装的封装的封装在体外 (in vitro) 的情况下,益生菌益生菌是一种益生菌.

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