从Rosa roxburghii中提取的自由和结合的多的净化,表征和抗炎潜力Tratt Pomace
Chao Li1, Hengyi Li1, Xiong Fu1
1Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Foods (Basel, Switzerland)
|July 13, 2024
概括
来自Rosa roxburghii Tratt果汁 (RRTP) 的多显示出强大的抗炎作用. 净化后的自由和结合的多醇有效地减少了炎症标记物,并抑制了巨细胞中的关键信号通路.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 罗莎·罗克斯堡希·特拉特 (RRTP) 是一个未充分利用的农业副产品,富含多.
- 多具有已知的抗氧化和抗炎性质,但它们来自RRTP的特定活动需要进一步调查.
研究的目的:
- 从RRTP中净化和表征自由多 (RRTP-FP) 和结合多 (RRTP-BP).
- 评估体外抗炎活性,阐明纯化RRTP多的分子机制.
主要方法:
- 用AB-8宏树脂提取和净化了多.
- 定量分析确定了主要的化合物.
- 在体外分析评估了抗氧化能力 (DPPH,ABTS),氧化 (NO) 和活性氧物种 (ROS) 生产以及LPS刺激的RAW264.7巨细胞中的细胞膜潜力.
- 分析了亲炎性细胞因子和iNOS的mRNA表达,以及NF-κB和MAPK信号通路.
主要成果:
- 净化显著增加了多醇含量.
- 发现的主要化合物包括表甲基素,表甲基素,酸和酸.
- 与维生素C相比,纯化的RRTP-FP和RRTP-BP表现出更高的抗氧化活性.
- 它们抑制了NO和ROS的产生,维持了细胞膜的潜能,并降低了促炎性细胞因子和iNOS的表达.
- 观察到NF-κB和MAPK信号通路的抑制.
结论:
- RRTP是强大的抗炎多的宝贵来源.
- 纯化的RRTP-FP和RRTP-BP通过调节关键信号通路,显示出显著的抗炎作用.
- 这些发现表明,RRTP衍生的多作为治疗炎症状况的治疗剂具有前景.
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