蛋白质:不同酸性和性环境对结构特征和功能性质的影响
Laijing Zhu1, Meng Liu1, Yanli Wang1
1School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Foods (Basel, Switzerland)
|July 13, 2024
概括
来自Euglena gracilis的微藻蛋白在食品应用中显示出有前途的功能性质. 它的结构和特性随着pH值的变化而变化,这表明它有可能用于乳液,凝和泡.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 微生物学 微生物学
背景情况:
- 对可持续蛋白质来源的需求日益增长.
- 微藻作为一种可行的替代蛋白质来源.
- 了解微藻蛋白结构和功能对于食品应用的重要性.
研究的目的:
- 为了从Euglena gracilis (EGP) 中提取蛋白质.
- 研究EGP的结构和功能性质.
- 分析EGP在不同酸性和性环境中的行为.
主要方法:
- 取和酸沉用于蛋白质分离.
- 尺寸排除色谱用于分子重量分布.
- 福里埃变换红外光谱法 (FTIR) 用于结构分析.
- 功能性质的分析 (发泡,乳化,溶解性等). ) 的情况.
- 对于晶度的X射线衍射 (XRD).
- 差分扫描热量计 (DSC) 用于测量热性能.
主要成果:
- EGP的分子重量在152至5.7kDa之间,富含疏水性和必需氨基酸.
- 结构分析 (FTIR) 显示,性pH的α-螺旋体含量较高,β-片含量较低.
- 在远离异电点的pH值下,功能性质 (溶解度,泡,乳化) 得到增强.
- 在酸性/性条件下,XRD表明晶体大小和结晶性减少.
- 在pH值11.0时,DSC显示了高化温度 (99.32°C) 和 (146.33 J/g).
结论:
- 优格伦花蛋白 (EGP) 在不同的pH水平上表现出显著的结构和功能变化.
- EGP显示出商业食品应用的潜力,特别是乳液,凝和泡.
- 该研究为利用EGP作为功能性食品成分提供了数据基础.
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