基于肝脏细胞毒性的基于基量子点的常见量子点替代品的比较
1Department of Biology, Missouri State University, 901 S National, Springfield, MO 65897, USA.
Nanomaterials (Basel, Switzerland)
|July 13, 2024
概括
添加碳点 (NCD) 在肝细胞中显示出量子点 (QD) 替代品中毒性最小. 其他替代品如InP/ZnS和CuInS2/ZnS也表现出一些毒性,与NCD不同.
科学领域:
- 纳米技术和材料科学 材料科学
- 生物医学工程 生物医学工程
- 毒理学 毒理学 毒理学
背景情况:
- 量子点 (QD),特别是硫化/硫化 (CdSe/ZnS),具有独特的光特性,在生物医学中具有价值,但存在毒性风险.
- 对传统的QD更安全的替代品对于推进生物医学应用和最大限度地减少不良健康影响至关重要.
- 肝脏是纳米颗粒的潜在积累地点,使肝细胞系成为毒性评估的适当模型.
研究的目的:
- 为了评估新出现的量子点替代品的体外毒性:酸/硫化 (InP/ZnS),铜酸/硫化 (CuInS2/ZnS) 和添加碳点 (NCD).
- 为了比较这些QD替代品在癌症 (HepG2) 和永生 (THLE-2) 的人类肝细胞系中的细胞反应.
- 调查毒性机制,包括细胞活力,活性氧物种 (ROS) 生成,亡和途径改变.
主要方法:
- 使用四染料 (XTT) 试验来评估细胞活力.
- 使用光成像检测反应性氧物种 (ROS) 生产.
- 进行了亡试验和RNA转录组学,以分析细胞损伤和途径调节.
主要成果:
- 在所有QD类型中,HepG2细胞在测试的纳米分子度 (10-150nM) 中没有显著降低活力.
- 在50-150nM度下,CdSe/ZnS和CuInS2/ZnS显著降低了THLE-2细胞活力,诱导了ROS早期 (6小时),并提高了亡 (24小时).
- RNA转录组学揭示了CdSe/ZnS,CuInS2/ZnS和InP/ZnS治疗的细胞中细胞粘附途径 (wnt,cadherin,integrin) 的下调,但不是在NCD治疗的细胞中.
结论:
- 添加碳点 (NCDs) 在两种肝细胞系中,在评估的QD替代品中表现出最低的毒性.
- 金属量子点 (InP/ZnS和CuInS2/ZnS),虽然毒性低于CdSe/ZnS,但在肝细胞中仍然存在潜在的毒性问题.
- 非传染性疾病是生物医学应用的有希望的更安全的替代品,需要进一步研究它们的长期细胞相互作用和疗效.
相关概念视频
¹H NMR Chemical Shift Equivalence: Homotopic and Heterotopic Protons
Protons in identical electronic environments within a molecule are chemically equivalent and have the same chemical shift. The replacement test is a useful tool to identify chemical equivalence and predict NMR spectra. A substituent replaces each of the protons being examined and the resulting molecules are compared. If the same molecule is obtained, the protons are equivalent or homotopic. Replacement of any hydrogens in ethane by chlorine yields chloroethane because all six protons are...
Pharmacokinetic Models: Comparison and Selection Criterion
Physiological and compartmental models are valuable tools used in studying biological systems. These models rely on differential equations to maintain mass balance within the system, ensuring an accurate representation of the dynamic processes at play.
Physiological models take a detailed approach by considering specific molecular processes. They can predict drug distribution, metabolism, and elimination changes, providing a comprehensive understanding of how drugs interact with the body.
Physiological models take a detailed approach by considering specific molecular processes. They can predict drug distribution, metabolism, and elimination changes, providing a comprehensive understanding of how drugs interact with the body.
Pharmaceutical Alternatives: Excipients and Impurities-Related Therapeutic Nonequivalence
Pharmaceutical products contain more than just the active drug; they also contain various excipients such as binders, solubilizers, stabilizers, preservatives, and other elements. In some cases, impurities or contaminants might be present. Traditionally, quality control in pharmaceuticals has primarily focused on the analysis of the active drug, often overlooking the impact of these additional components. The recent issue with heparin contamination by over-sulfated chondroitin sulfate, a...
Pharmaceutical Alternatives: Polymorphic Form-Related and Particle Size-Related Therapeutic Nonequivalence
Changes in polymorphic forms can significantly influence the bioavailability of poorly soluble drugs. Although the FDA defines pharmaceutical equivalence based on having the same active ingredient, dosage form, and route of administration, it does not automatically disqualify products with different polymorphic forms. This means two products with different polymorphs can still be deemed pharmaceutically equivalent. However, polymorphic differences can affect properties like wettability,...


