相关实验视频

Updated: Jun 21, 2025

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Published on: September 1, 2017

在植物中通过定制的2D-RP-HPLC分离与ICP和ESI MS检测揭示新的化合物

Aleksandra Izdebska¹, Sylwia Budzyńska², Katarzyna Bierla¹

¹Université de Pau et des Pays de l'Adour, E2S UPPA, CNRS, IPREM UMR 5254, Helioparc, 64053 Pau, France.

Molecules (Basel, Switzerland)

|July 13, 2024

概括

这项研究开发了一种分析植物中 (As) 化合物的新方法,识别了以前未被检测到的As-phytochelatins和arseno脂质. 这有助于我们更好地了解.

科学领域:

环境化学环境化学
植物生物学植物生物学
分析化学分析化学

背景情况:

(As) 物种化对于理解其对环境的毒性和生物效应至关重要.
传统方法往往无法检测植物样本中的所有相关As化合物.
代谢活性As化合物可以使用标准分析技术保持未识别.

研究的目的:

开发和优化一种全面的方法,用于植物基质中的物种分析.
在暴露于的植物中识别以前未被检测到的或较小的化合物.
提高对植物中代谢和积的理解.

主要方法:

探索各种提取程序和高性能液体染色学 (HPLC) 方法.
开发固体相提取方法,用于样品净化和预缩.
在线2D逆相HPLC (RP-HPLC) 系统与诱导合等离子体 (ICP) 和电喷离子质谱 (ESI-MS) 结合实施.

主要成果:

阳离子交换染色学只确定了1982%的总As,留下了大量未被检测到的部分.
新的2D-RP-HPLC方法成功地检测并识别了暴露于As(III) 的幼苗中的As(III) -植物甲基和arseno-thio化合物.
在暴露于As(V) 的植物根部中发现了arseno脂质.

结论:

开发的2D-RP-HPLC方法为植物中的物种形成提供了更全面的方法.
这种方法可以检测以前被忽视的,可能是代谢相关的化合物.
这些发现有助于更好地了解植物中的吸收,转化和毒性.

关键词:

2D RP分离方式在ICP-MS中,使用的是ICP-MS.基于MS/MS的识别方法种类的变化种类的变化

更多相关视频

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Published on: October 2, 2016

Removal of Arsenic Using a Cationic Polymer Gel Impregnated with Iron Hydroxide

Removal of Arsenic Using a Cationic Polymer Gel Impregnated with Iron Hydroxide

Published on: June 28, 2019

相关概念视频

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...

Atomic Emission Spectroscopy: Lab

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...