通过优化辅因子PLP和NADPH合成来增加大肠杆菌中的1,4-氨基butan产量
Tong Sun1,2, Yongcan Zhao1,2, Jinjin Wang1,2
1Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457, China.
Molecules (Basel, Switzerland)
|July 13, 2024
概括
通过增加 Escherichia coli 中的皮里多酸盐 (PLP) 和 NADPH 的细胞水平,增强了 1,4-diaminobutane 的微生物生产. 这种代谢工程方法使得1,4-diaminobutane的产量增加了79%.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 1,4-氨基是一种有价值的工业化学品,可用于聚合物,制药和农业化学品.
- 微生物生产为传统合成提供了一个经济和环境可持续的替代方案.
- 有效的微生物合成需要了解和优化辅助因子的要求,特别是酸 (PLP) 和NADPH.
研究的目的:
- 为了提高1,4-diaminobutane的微生物合成效率.
- 调查氧化酸盐 (PLP) 和NADPH对大肠杆菌中1,4-二氨基产生的影响.
- 优化大肠杆菌中PLP和NADPH生物合成的代谢途径.
主要方法:
- 大肠杆菌的基因工程,以过度表达参与PLP和NADPH合成的基因.
- 用葡萄糖作为主要碳来源培养复合菌株.
- 量化1,4-亚米诺布坦产量和细胞辅因子水平.
主要成果:
- 优化的基因表达导致了PLP和NADPH细胞内度的增加.
- 经过工程改造的NAP19菌株实现了1,4-亚米诺布坦272 mg/L·DCW的产量.
- 这代表了79%的收益率增加,与家长的底盘压力相比.
结论:
- 提高细胞PLP和NADPH水平是一种有效的策略,可以增强微生物的1,4-diaminobutane生产.
- 对辅助因子生物合成途径的代谢工程可以显著提高有价值化学品的产量.
- 开发的重组菌株显示出对工业规模的1,4-diaminobutane可持续生产的希望.
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