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通过单细胞转录组分析识别三阴性乳腺癌中新化学抵抗相关基因

Spyros Foutadakis¹, Dimitrios Kordias^2,3, Giannis Vatsellas¹

¹Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.

International journal of molecular sciences

|July 13, 2024

概括

单细胞测序揭示了罕见的三阴性乳腺癌细胞驱动化学抵抗. 像EMT一样识别这些细胞和途径为针对这种攻击性癌症的个性化治疗提供了新的目标.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
细胞生物学细胞生物学

背景情况:

三阴性乳腺癌 (TNBC) 是由于耐化疗而具有不良结果的侵略性.
目前的治疗方法面临的挑战是TNBC的药物耐药性.
了解TNBC异质性对于改善治疗策略至关重要.

研究的目的:

调查TNBC细胞在发展化疗抵抗之前和之后的转录差异.
为了比较大量RNA测序和单细胞RNA测序 (scRNA-seq) 在识别抵抗机制.
发现TNBC中克服化疗抵抗的新型治疗点.

主要方法:

在TNBC细胞上进行了大量RNA测序和scRNA-seq.
细胞被培养成2D单层和3D球形.
使用帕克利塔塞尔和多克索鲁比辛诱导了耐药性.

主要成果:

在TNBC群体中观察到显著的转录异质性.
scRNA-seq发现了大量测序中遗漏的罕见耐化细胞子集.
耐化学性细胞显示了部分转向介质细胞表型的转变,表明EMT.

结论:

单细胞方法对于剖析瘤异质性和识别罕见的耐药细胞至关重要.
皮质转介质转换 (EMT) 是TNBC亚组中化学抵抗的一个关键机制.
在EMT中涉及的PDGF信号传递等向途径可能为TNBC提供新的治疗途径.

关键词:

3D球形形状的球形体.大量的RNA测序.化学电阻是一种化学电阻.一个单细胞RNA测序的序列.翻译学翻译学翻译学翻译学三重阴性乳腺癌是什么

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