绘制人类精子生成的发展使用转录基数据:一个范围审查.
Lena Kwaspen1, Marc Kanbar1,2, Christine Wyns1,2
1Laboratoire d'Andrologie, Pôle de Recherche en Physiologie de la Reproduction, Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium.
International journal of molecular sciences
|July 13, 2024
概括
试管成熟 (IVM) 显示出恢复生育能力的前景. 本综述详细介绍了人类丸的发育和成熟,并确定了优化IVM协议以获得更好的结果的关键因素.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 试管成熟 (IVM) 为非阻塞性精子和青春期前男孩提供生育恢复.
- 人体体外精子生成仍然具有挑战性,需要对丸发育有更深入的了解.
- 在人类丸发育过程中,转录分析对优化IVM至关重要.
研究的目的:
- 描述人类丸的发育和成熟,从胎儿到成人的阶段.
- 为了确定影响丸成熟的因素,以优化IVM协议.
- 对使用scRNA-seq.q.培养的原生和体外培养人类丸细胞的现有文献进行审查.
主要方法:
- 对人类丸细胞 (原生和体外) 的研究论文进行范围审查.
- 对单细胞RNA测序 (scRNA-seq) 数据的分析.
- 专注于基因本体学术语来解释基因功能.
主要成果:
- 人体丸成熟涉及11岁左右的显著转录性变化.
- scRNA-seq数据建议重新评估精子干细胞 (SSC) 状态 (A 暗/白).
- 对体细胞的数据,特别是对塞尔托利细胞的数据是有限的.
- 在体外培养有利于无差异的SSC与AKT信号通路抑制剂.
- 氧化酸化途径的参与取决于成熟状态.
结论:
- 了解丸的发育和成熟为改善IVM提供了洞察力.
- 在发育过程中确定的关键细胞信号通路可能对IVM成功至关重要.
- 需要对体细胞和精细的SSC分类进行进一步的研究,以实现IVM的进步.
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