在口腔状细胞癌中,剪接机械受损,并且与关键的病理生理特征有关
Alba Sanjuan-Sanjuan1,2,3,4, Emilia Alors-Perez1,2,5,6, Marina Sanchez-Frías1,2,7
1Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain.
International journal of molecular sciences
|July 13, 2024
概括
替代拼接机械 (SM) 在口腔状细胞癌 (OSCC) 中失调. 用Pladienolide-B抑制SM活动减少了OSCC细胞的增殖,这表明了这种癌症的潜在新疗法.
科学领域:
- 分子瘤学分子瘤学
- 癌症基因组学 癌症基因组学
- 生物化学 生化学
背景情况:
- 替代拼接失调被认为是癌症的标志.
- 拼接机械 (SM) 组件是癌症治疗的潜在目标.
- 口腔状细胞癌 (OSCC) 是一种普遍存在的头癌,具有未满足的治疗需求.
研究的目的:
- 在OSCC中描述拼接机械 (SM) 部件的表达.
- 调查SM成分表达和临床/病原学特征之间的关联.
- 评估OSCC中SM抑制的治疗潜力.
主要方法:
- 未来病例控制研究分析了37名OSCC患者瘤和健康组织中的59个SM成分.
- 对SM成分表达的相关性分析与临床和本病理学数据.
- 在实验室中对Pladienolide-B (SM抑制剂) 对OSCC细胞增殖的评估.
主要成果:
- 在OSCC中,与相邻的健康组织相比,观察到59个SM组件中的12个 (20%) 的显著失调.
- 改变的SM成分表达与不那么积极的临床特征和改善的整体存活率相关.
- 普拉迪埃诺利德-B治疗显著降低了初级OSCC细胞培养的增殖率.
结论:
- 拼接机械失调是OSCC的一个重要特征,与疾病特征和患者的结果有关.
- 通过Pladienolide-B证明的拼接机械活动的抑制,对OSCC细胞表现出直接的抗增殖作用.
- 准拼接机器是口腔状细胞癌的有希望的治疗策略.
相关概念视频
RNA Splicing
56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Alternative RNA Splicing
21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
Loss of Tumor Suppressor Gene Functions
4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Mismatch Repair
4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K
Induced Pluripotent Stem Cells
4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.0K


