相关实验视频

Updated: Jun 21, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

白三叶草TrMYB33-TrSAMS1模块通过通过ABA依赖途径调节精子胺生物合成,促进干旱耐受性

Youzhi Zhang¹, Xiaofang Qin¹, Zhirui He¹

¹College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

International journal of molecular sciences

|July 13, 2024

概括

这项研究揭示了Trifolium repens TrMYB33如何通过控制TrSAMS1基因表达来调节干旱诱导的精子产量,通过酸信号增强植物干旱耐受性.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
生物化学生化学

背景情况:

干旱下的植物中精氨酸的积累已知,但其调节网络和分子机制尚不清楚.
酸 (ABA) 参与植物应激反应,包括干旱耐受性.

研究的目的:

阐明干旱诱导的三叶草植物中的精子胺生物合成和积累背后的分子机制.
确定干旱压力下的精子胺生产中涉及的关键调节因素.

主要方法:

转录组和表达分析以确定候选基因.
酵母的一种混合查以识别相互作用的蛋白质.
基因过度表达和VIGS介导的沉默来确定基因功能.
分析ABA在精子蛋白积累中的作用.

主要成果:

确定了TrMYB33作为一个关键的调节器,该调节器将ABA信号传递给干旱诱导的精子胺生产.
编码S-adenosylmethionine合成酶的TrSAMS1被确定为TrMYB33.3的直接标基因.
TrSAMS1积极调节精氨酸的合成和耐旱性.
TrMYB33直接与TrSAMS1促进体结合,并作为转录激活剂.
在干旱压力下,精子胺的积累依赖于ABA,并由TrMYB33-TrSAMS1模块介导.

结论:

TrMYB33-TrSAMS1调节模块在干旱诱导的三叶草植物中的精子胺生物合成和积累中发挥着至关重要的作用.
这一发现增强了对植物干旱应激反应机制的理解.
TrMYB33-TrSAMS1模块提供了提高作物耐旱能力的潜在目标.

关键词:

在ABA中依赖于ABA.转录因子MYB转录因子这是一种S-adenosylmethionine合成酶.抗旱能力抗旱能力抗旱能力精子中的精子.白色的三叶草是白色的

更多相关视频

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Published on: July 20, 2019

相关概念视频

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.