对2型糖尿病患者循环miRNA表达特征的分析 糖尿病足并发症患者
Giovanny Fuentevilla-Alvarez1, María Elena Soto2,3, Gustavo Jaziel Robles-Herrera4
1Endocrinology Department, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1. Col. Sección XVI, Mexico City 14080, Mexico.
International journal of molecular sciences
|July 13, 2024
概括
像miR-17,miR-191和miR-121这样的微RNA (miRs) 在糖尿病足患者中升高,与较高的葡萄糖水平相关. 这些miRs显示出作为糖尿病并发症生物标志物的潜力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 2型糖尿病 (T2DM) 的并发症,如糖尿病足,对健康构成重大风险,包括感染和截肢.
- 目前用于糖尿病足的治疗方法的疗效有限,这凸显了需要新的治疗策略的需要.
- 微RNAs (miRs) 是基因表达的关键调节者,并且越来越多地被认为是它们在伤口愈合和糖尿病发病过程中的作用.
研究的目的:
- 研究2型糖尿病 (T2DM) 和糖尿病足 (DFU) 患者的特定微RNA (miRs) 的差异表达.
- 探索高MIR水平与T2DM患者的血糖控制之间的相关性.
- 评估已识别的miRs作为糖尿病足并发症生物标志物的诊断潜力.
主要方法:
- 从41名墨西哥T2DM和DFU患者和50名健康对照患者收集了血样本.
- 使用定量逆转录聚合酶连锁反应 (RT-qPCR) 来分析选定的miRs的表达水平.
- 进行了统计分析,包括相关性和接收器运行特征 (ROC) 曲线分析,以评估结果.
主要成果:
- 与对照组相比,DFU患者的miR-17-5p,miR-191-5p,let-7e-5p和miR-33a-5p的血水平显著更高 (所有患者的p<0.005).
- 在DFU患者中,miR-17,miR-191和miR-121的升高水平与更高的血糖水平呈正相关性 (r = 0.30至0.21,p ≤0.05).
- miR-17在糖尿病足的诊断准确度最高,曲线下的面积 (AUC) 为0.903 (p = 0.0001).
结论:
- 特定的微RNA,包括miR-17,miR-191和miR-121,在患有糖尿病足的患者中显著上调.
- 这些上调的miR与血糖控制不佳密切相关,这表明在T2DM并发症的发展中发挥了作用.
- 已识别的miRs,特别是miR-17,作为潜在的诊断生物标志物,有望为糖尿病足并发症的早期检测和管理提供希望.
更多相关视频
11:44Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
7.6K
06:48Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
Published on: April 29, 2022
2.3K
相关概念视频
01:26Type I Diabetes II: Pathophysiology
Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular uptake of...
01:26Type II Diabetes I: Introduction
Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance, in which target tissues such as the liver, muscle, and adipose tissue respond poorly to insulin. It is also associated with inadequate compensatory insulin secretion, where pancreatic β-cells fail to produce sufficient insulin. Together, these abnormalities lead to persistent hyperglycemia.EtiologyT2DM develops through a complex interaction of genetic predisposition and environmental or...
01:24Type II Diabetes II: Pathophysiology
PathophysiologyType 2 diabetes mellitus (T2DM ) is a chronic metabolic disorder characterized by insulin resistance and progressive pancreatic β-cell dysfunction, leading to impaired glucose homeostasis. It results from interactions among genetic predisposition, environmental factors, and metabolic stressors, such as overnutrition and a sedentary lifestyle.Insulin Resistance and Glucose DysregulationEarly T2DM involves insulin resistance in skeletal muscle, adipose tissue, and the liver.
01:25Type II Diabetes Mellitus III: Clinical Manifestations and Diagnosis
Type 2 diabetes mellitus develops gradually and is often asymptomatic in early stages.Clinical ManifestationsWhen symptoms appear, they include fatigue, blurred vision, pruritus, delayed wound healing, and recurrent infections, particularly candidal infections. Peripheral neuropathy may present as numbness or tingling in the extremities. Classic hyperglycemia symptoms—polyuria, polydipsia, and polyphagia—are less common. Most patients are overweight and frequently have associated hypertension...
01:31Diabetic Foot Ulcer
Definition A diabetic foot ulcer (DFU) is a chronic, non-healing wound that develops in individuals with diabetes. It typically occurs on pressure-bearing areas such as the heel, metatarsal heads, or hallux, and carries a high risk of infection and amputation.Pathophysiology • The development of DFUs can be explained by four interconnected mechanisms: neuropathy, ischemia, infection, and impaired wound healing. • Neuropathy is the most common factor. Sensory neuropathy reduces pain perception,...
01:27Diabetic Retinopathy
DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...