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使用自动化处理系统用原酶隔离流体血管部分的试点研究.

Gershon Zinger1, Yoav Gronovich2, Adi Maisel Lotan2

  • 1Department of Orthopedic Surgery, Hand Unit, The Eisenberg R&D Authority, Shaare Zedek Medical Center, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9103102, Israel.

International journal of molecular sciences
|July 13, 2024
PubMed
概括

自动化细胞站 (ACS) 从脂肪组织中有效地隔离了无菌的脂肪衍生的树皮细胞. 这个系统为治疗应用提供了有价值的树皮血管分数 (SVF) 来源.

关键词:
在ACS ACS中使用.这些ASC是ASCs.在SVF中使用SVF.脂肪组织的脂肪组织.细胞计数细胞计数的数量.再生细胞的再生细胞.流体血管分数 流体血管分数

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科学领域:

  • 再生医学是一种再生医学.
  • 细胞生物学 细胞生物学
  • 生物技术是生物技术.

背景情况:

  • 自主脂肪衍生的树皮细胞 (ASCs) 具有显著的治疗潜力.
  • 这些细胞是从脂肪组织中分离出来的体血管分数 (SVF) 的一部分.
  • 闭式自动化系统提供了一种从脂肪组织中分离细胞的方法.

研究的目的:

  • 评估自动化细胞站 (ACS) 的产量,纯度和SVF的细胞特征.
  • 为了评估孤立的SVF的茎度标准和无菌性.
  • 为了确定ACS系统在处理脂肪组织用于细胞治疗的效率.

主要方法:

  • 使用自动化细胞站 (ACS) 从三个捐赠者中分离了SVF.
  • 鉴定包括细胞产量,活力,免疫型 (流细胞计),分化潜力和殖民地形成单位.
  • 通过对内毒素和原酶残留物的测试来评估无菌性.

主要成果:

  • 该ACS系统平均产生了7.9毫升的SVF与19 × 10^6核细胞在85%的生存率.
  • 免疫类型检测显示出异质细胞群,包括ASC (23%),巨细胞 (24%) 和内皮细胞 (5%).
  • 该SVF表现出多能分化能力 (adipogenic,chondrogenic,osteogenic),并且没有内毒素和原酶残留物.

结论:

  • 该ACS系统在单次手术过程中高效地处理脂肪组织.
  • 该系统产生了无菌的,缩的SVF,富含ASCs,适合治疗用途.
  • 这种自动化方法为再生医学应用提供了可靠的细胞来源.