在深层阳性溶剂中4'-氧的生物还原:优化和有效性与各种酵母菌株

在深层阳性溶剂中4'-氧的生物还原:优化和有效性与各种酵母菌株

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Paweł Chlipała¹, Tomasz Janeczko¹, Marcelina Mazur¹

¹Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.

International journal of molecular sciences

|July 13, 2024

概括

本研究探讨使用酵母生物催化剂在深层浸泡溶剂 (DESs) 中生产4'-二甲. 某些酵母菌株的转化率超过95%,证明了这种绿色化学方法的潜力.

科学领域:

生物催化和绿色化学
自然产品的合成自然产品的合成
微生物生物技术微生物生物技术

背景情况:

4'-二甲是药用植物和龙血树脂中发现的生物活性化合物.
它们的合成为开发新的治疗剂提供了机会.
生物催化剂为化学转化提供了一个可持续的替代方案.

研究的目的:

为了研究酵母生物催化在深溶解剂 (DESs) 中用于生产4'-二甲的使用.
为了确定最佳条件和适合的酵母菌株这种生物转化.
评估DESs作为酵母催化减少反应介质的效率.

主要方法:

培养酵母菌株,包括*Yarrowia lipolytica*,*Rhodotorula*和*Debaryomyces*.这些菌株的种植.
生物还原过程在各种深度浸泡溶剂 (DES) 中进行,其中糖醇作为键供体 (HBD).
优化反应温度,含水量和基质度.

主要成果:

35°C的最佳温度和含水量为50%的DES在1小时内产生了高转换率 (>70%).
酵母菌株*Rhodotorula marina* KCh 77和*Rhodotorula rubra* KCh 4表现出极好的生物催化活性,实现了超过95%的转化.
基质度增加导致转化效率下降.

结论:

酵母菌株是有效的生物催化剂,用于选择性降低α,β不和.
深度浸泡溶剂 (DES) 是适合和有效的介质,用于酵母介导的4'-二甲的合成.
这项研究突出了自然产品合成的有希望的绿色化学策略.

关键词:

雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.生物催化剂的生物催化剂石灰石是指石灰石块的组成部分.二甲基基的二甲基.

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