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揭示PheCLE1和PheCLE10 基于全基因组分析促进根部发育

Changhong Mu¹, Wenlong Cheng¹, Hui Fang¹

¹Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing 100102, China.

International journal of molecular sciences

|July 13, 2024

概括

研究人员在Moso竹中发现了11个CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) 基因. 这些基因,特别是PheCLE1和PheCLE10,对于顶组织发育至关重要,并在大米中过度表达时促进根生长.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
发育生物学是发展生物学.

背景情况:

莫索竹的快速生长与其角性水系动力学有关.
CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) 基因在模型植物中调节水系和器官的形成.
目前尚不清楚CLE基因在Moso竹中的功能.

研究的目的:

为了在Moso竹中进行全基因组CLE基因的识别.
研究莫索竹CLE基因的基因结构,染色体定位,进化关系和表达模式.
探索特定的CLE基因在根部发育中的作用.

主要方法:

在Moso竹中全基因组识别CLE基因.
对基因结构,染色体定位和进化关系的分析.
基因表达的转录和单细胞转录分析.
在现场杂交以确认基因表达模式.
在大米中进行过度表达研究,以评估功能性作用.
对转录因子与基因促进体结合的分析.

主要成果:

确定了11个Moso竹CLE (PheCLE) 基因,每个基因都有一个保存的CLE基因.
PheCLE基因分布在不同的群体中,在米和玉米中与CLE基因发生合成.
PheCLE基因在莫索竹的顶端组织中显著表达,PheCLE1和PheCLE10在基底根尖高度表达.
在大米中过度表达PheCLE1和PheCLE10显著增强了根生长.
PheCLE1和PheCLE10位于细胞膜上,由NAC9和NAC6转录因子激活.

结论:

这项研究首次系统地识别了Moso竹中的CLE基因家族.
PheCLE 基因在 Moso 竹的脊柱组织发育中起着至关重要的作用.
PheCLE1和PheCLE10是根生长的关键调节者,为竹子中的信号网络提供了洞察力.

关键词:

在CLE基因家族的基因家族.莫索竹的竹子可以使用.这就是PheCLE1的原因.在PheCLE10中,PheCLE10是状组织的发育.

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