苦味受体46 (hTAS2R46) 保护单细胞/巨细胞免受氧化应激的影响
Maria Talmon1, Lara Camillo2, Ilaria Vietti2
1Department of Pharmaceutical Sciences, University of Piemonte Orientale, 28100 Novara, Italy.
International journal of molecular sciences
|July 13, 2024
概括
苦味受体 (TAS2Rs) 保护人类单细胞/巨细胞DNA免受氧化应激. 阿布辛丁是一种TAS2R46激动剂,可减少DNA损伤和活性氧/物种,支持免疫细胞功能.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 化学感应是一种化学感应.
背景情况:
- 苦味受体 (TAS2Rs) 超越了味觉感知,形成了一个全身的化学感知系统.
- TAS2Rs在人类免疫细胞中表达,包括单细胞/巨细胞,在那里它们调节先天免疫反应.
- 氧化应激可以损害单细胞/巨细胞的功能,导致细胞死亡,尽管它们在病原体防御中的作用.
研究的目的:
- 研究TAS2R46对人类单细胞和巨细胞在压力引起的DNA损伤中的保护作用.
- 确定TAS2R46激活是否可以减轻这些免疫细胞中的氧化和化应激.
主要方法:
- 利用直接和间接测试来评估DNA损伤.
- 采用单细胞凝电泳试验来量化DNA完整性.
- 给人体单细胞/巨细胞培养物注射了absinthin,一种特定的TAS2R46激动剂.
主要成果:
- 阿布辛丁通过TAS2R46起作用,显著抵消了活性氧物种 (ROS) 和活性物种 (RNS) 的释放.
- TAS2R46激活显然可以减少单细胞和巨细胞中压力诱导的DNA损伤.
- 这些发现表明,在保护细胞免受氧化损伤的同时,它在保护抗菌功能方面发挥了双重作用.
结论:
- TAS2R46被确定为单细胞和巨细胞对氧化应激的新型保护因子.
- 激活TAS2R46为缓解免疫细胞损伤提供了一个潜在的治疗点.
- 这项研究强调了TAS2Rs在维护免疫系统完整性和功能方面的更广泛作用.
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