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转录组分析揭示了 Plesiomonas shigelloides 中的鞭毛转录层次和分泌系统之间的交叉对话

Junxiang Yan^1,2,3, Zixu Zhang^1,2,3, Hongdan Shi^1,2,3

¹TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China.

International journal of molecular sciences

|July 13, 2024

概括

这项研究确定了四个关键调节剂 (FlaK,Flam,FliA和FliA_L) 控制Plesiomonas shigelloides的鞭毛基因表达. 这些调节器影响运动,毒性和代谢活动,揭示了鞭毛系统和分泌系统之间的交叉对话.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌病原体的产生

背景情况:

普莱西莫纳斯·希格洛伊德 (Plesiomonas shigelloides) 是一种格拉姆阴性细菌,它会导致人类的胃肠道和肠外疾病.
在P. shigelloides中,鞭毛转录层次结构在很大程度上仍然没有被描述.
了解鞭毛调节对于阐明P. shigelloides毒性机制至关重要.

研究的目的:

为了识别和描述P. shigelloides中极性和侧面鞭毛的调节者.
阐明鞭毛转录层次结构及其控制机制.
为了研究鞭毛细胞调节,毒性和代谢活动之间的相互作用.

主要方法:

野生型 (WT) 和突变菌株 (ΔflaK, ΔflaM, ΔfliA, ΔfliA_L) 的转录组分析 (RNA-seq).
定量实时聚合酶连锁反应 (qRT-PCR) 和发光选试验用于验证.
电泳移动转移试验 (EMSA) 用于确定直接的DNA-蛋白质结合相互作用.

主要成果:

确定了四种调节器:FlaK,Flam,FliA和FliA_L,它们控制着鞭毛基因表达.
FlaK直接与fliK,fliE,flhA和cheY的促进体结合;Flam与flgO,flgT和flgA的促进体结合.
观察到VI型和II型分泌系统的下调;在突变菌株中减少细菌杀死和Caco-2细胞入侵.
在鞭毛调节器突变体中,生理代谢基因的差异表达.

结论:

在P. shigelloides中建立了鞭毛转录层次结构,包括Flak,Flam,FliA和FliA_L.
证明了鞭毛层次结构,细菌分泌系统和毒性之间的交叉交谈.
突出了鞭毛调节器对细菌生理学,新陈代谢和宿主-病原体相互作用的影响.

关键词:

这种植物是Plesiomonas shigelloides.在RNA-seqqq.在T2SS-2中使用.在T6SSSS中使用.交叉谈话交叉谈话交叉谈话旗的转录层次结构.生理和代谢的生理和代谢.

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