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Skin Cancer01:30

Skin Cancer

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Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
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Raman Spectroscopy: Overview01:20

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Updated: Jun 21, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

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恶性皮肤新生体的多波长拉曼分化.

Elena Rimskaya1, Alexey Gorevoy1, Svetlana Shelygina1

  • 1Lebedev Physical Institute, 119991 Moscow, Russia.

International journal of molecular sciences
|July 13, 2024
PubMed
概括

拉曼显微镜分辨皮肤癌使用光谱特征. 这项技术分析了健康的基底细胞癌 (BCC) 和状细胞癌 (SCC) 组织中的分子差异,提供了一个非侵入性诊断工具.

关键词:
拉曼显微光谱法 拉曼显微光谱法基底细胞癌的癌症.多光谱分析的方法状细胞癌 (SCLC) 是一种状细胞癌.

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科学领域:

  • 生物医学光学 生物医学光学
  • 分子光谱学 分子光谱学
  • 皮肤病学 皮肤病学

背景情况:

  • 拉曼显微镜是一种强大的技术,用于对生物组织进行分子分析.
  • 区分健康和恶性皮肤组织,如基底细胞癌 (BCC) 和状细胞癌 (SCC),对于有效的治疗至关重要.

研究的目的:

  • 调查体外拉曼光谱法在区分健康,BCC和SCC皮肤组织中的有用性.
  • 为了确定特定的光谱特征和最佳的激发波长,以便进行准确的分类.

主要方法:

  • 通过532nm和785nm激光激发,从健康的皮肤组织,BCC和SCC中获得体外拉曼光谱.
  • 分析900-1800厘米-1和2800-3100厘米-1波数范围内的光谱数据.
  • 评估特定拉曼波段的强度比,以进行差异化.

主要成果:

  • 波段的强度比在1268,1336和1445cm-1在532nm有效地区分了三个样本类型.
  • 波数较高的波段 (2850,2880,2930厘米−1) 在两种波长的瘤中表明蛋白质/脂质比增加.
  • 将两种激发波长的数据结合起来,实现了87%的准确性,用于三类差异化.

结论:

  • 多波长拉曼光谱为分析恶性皮肤瘤中的分子变化提供了一种多功能,非侵入性的方法.
  • 这种技术对皮肤癌过程和潜在的其他癌症的研究有希望.
  • 优化的光谱分析可以显著提高皮肤癌检测的诊断准确性.