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在Schima superba中对DIR基因家族的全基因组识别,进化和表达分析

Changya Chen^1,2, Yanling Cai², Boxiang He²

¹School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.

International journal of molecular sciences

|July 13, 2024

概括

研究人员在中国树中发现了24个Dirigent (DIR) 基因,揭示了它们在植物应激反应和素生物合成中的作用. 这项研究提高了对植物适应机制的理解.

科学领域:

植物分子生物学植物分子生物学
林业科学林业科学
生物化学生物化学

背景情况:

中国古格树 (Schima superba) 在中国南部的常青森林中具有生态意义.
导向蛋白 (DIR) 对素/素合成,二次新陈代谢和抗压能力至关重要.
在S. superba.中对DIR基因家族的研究有限.

研究的目的:

在Schima superba.中识别和描述DIR基因家族.
研究SsDIR基因的进化和功能作用.
探索SsDIR基因在干旱应激反应中的潜在参与.

主要方法:

用于基因识别和分类的生物信息分析.
染色体分布和对线性分析.
基因表达在不同的组织和干旱压力下进行分析.
对于cis-regulatory元素的推动者分析.

主要成果:

确定了24个SsDIR基因,分为三个子家族.
SsDIR基因在染色体上分布不均,其中83%缺乏内核.
双重复制是基因家族扩张的主要驱动力.
观察到明显的组织特异性表达模式,具有高根表达.
几个SsDIR基因在干旱压力下显示出差异性表达.
促进区域包含对发育,荷尔蒙和压力的 cis 调节元素.

结论:

斯迪尔基因家族主要通过并列重复而经历了扩张.
SsDIR基因表现出组织特异性表达,并参与压力反应,特别是干旱.
鉴定到的 cis-regulatory 元素表明了不同的监管功能.
这项研究为了解S. superba.中的红素/红素生物合成和抗压力提供了基础.

关键词:

斯基马的超级宝贝无生物压力是非生物压力.导演家族是指导家庭.基因表达分析基因表达分析进行全基因组分析.

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