相关实验视频
Updated: Jun 21, 2025

09:58
Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
11.0K
增强过氧化分解在连续流动反应器中,与PAES-C的固定催化酶相比
Yunrui Li1, Yu Zhang1, Wenyu Zhang1
1College of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China.
Polymers
|July 13, 2024
概括
在一种新型聚乙烯硫载体 (PAES-C) 中的固定性催化酶显示出增强的活性和稳定性. 这种绿色化学方法为工业应用提供了更好的酶可重复使用性.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 材料科学 材料科学 材料科学
- 绿色化学 绿色化学
背景情况:
- 酶催化提供了高特异性和效率,引发了对工业酶应用的兴趣.
- 酶固定增强了稳定性和可回收性,保护酶免受恶劣环境的影响.
- 开发高效的固定载体对于扩大酶功效至关重要.
研究的目的:
- 为了将催化酶固定在具有可调节性质的聚乙烯硫载体 (PAES-C) 上.
- 为最大载体和酶值优化固定参数.
- 在模型反应中评估固定性催化酶的性能.
主要方法:
- catalase被固定在合成的聚乙烯硫载体 (PAES-C) 上.
- 固定条件 (温度,时间,酶剂量) 被优化.
- 测量了酶活性,热稳定性和吸附能力.
- 在连续流动反应堆中进行过氧化分解,以评估性能.
主要成果:
- 与自由酶相比,固定性催化酶表现出更高的活性 (188.75 U/g) 和60 °C的热稳定性.
- 这种载体表现出高酶蛋白吸附能力,为4.685 mg/g.
- 固定化的酶在连续流系统中实现了90%的转化率.
- PAES-C合成利用了可再生资源,与绿色化学原则保持一致.
结论:
- PAES-C是一种有效的载体,可以固定催化酶,提高其催化性能和稳定性.
- 固定化简化了酶分离,提高了可重复使用性.
- 开发的PAES-C载体通过绿色化学合成支持可持续的酶技术.
相关概念视频
Catalysis
26.8K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
26.8K
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
3.3K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.3K
Catalytically Perfect Enzymes
4.0K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Most enzymes...
Most enzymes...
4.0K
Reduction of Alkenes: Catalytic Hydrogenation
12.0K
Alkenes undergo reduction by the addition of molecular hydrogen to give alkanes. Because the process generally occurs in the presence of a transition-metal catalyst, the reaction is called catalytic hydrogenation.
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
12.0K
Peroxisomes
11.8K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
11.8K
Esters to Carboxylic Acids: Acid-Catalyzed Hydrolysis
2.8K
Hydrolysis of esters under acidic conditions proceeds through a nucleophilic acyl substitution. In the presence of excess water, the reaction proceeds in a reversible manner, forming carboxylic acids and alcohols.
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
2.8K

