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在玉米IBM Syn10 DH种群中对灰叶斑抗性的定量特征局部分析

Lina Cui^1,2, Mingfei Sun³, Lin Zhang⁴

¹Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|July 13, 2024

概括

研究人员确定了QTL和用于玉米灰叶斑抗性的候选基因. 这一发现有助于开发抗性玉米杂交品种,这对作物产量和降低生产成本至关重要.

科学领域:

植物病理学植物病理学
遗传学是一个遗传学.
农业科学农业科学

背景情况:

由Cercospora zeae-maydis和Cercospora zeina引起的灰色叶斑 (GLS) 是一种严重的真菌病,影响全球玉米生产.
开发耐药的玉米杂交品种是减轻经济损失和确保粮食安全的关键.
抗GLS是一种复杂的特征,受多种基因和环境因素的影响.

研究的目的:

确定与玉米灰叶斑抗性相关的定量特征位点 (QTL).
通过基因组和转录组分析,精确定位参与灰叶斑抗性的候选基因.
在玉米育种计划中为标记器辅助选择提供资源.

主要方法:

利用IBM Syn 10双倍哈普洛伊德 (DH) 群体在七个环境中进行QTL分析.
综合转录组数据和父系之间的基因组结构变异.
将已识别的QTL与现有的GWAS位点进行比较,并分析了耐药性域的候选基因.

主要成果:

发现了58个GLS耐药性的QTLs,其中49个在染色体1,2,3,4,8和10上形成了12个群.
确定了110个具有显著表达和父系之间结构差异的基因.
确定了19个潜在的候选基因,包括编码富含白的重复受体和NLP转录因子的基因.

结论:

已识别的QTL和候选基因为加速玉米灰叶斑抗性繁殖提供了有价值的目标.
这项研究为标记器辅助选择策略提供了链接的位置和基因组资源.
了解GLS耐药性的遗传基础对于开发可持续农业实践至关重要.

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