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Updated: Jun 21, 2025

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
14:27

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity

Published on: August 19, 2013

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协议使用蛋白质语言模型预测和实验验证后提胺-N-糖氨酸酶的功能增强变体

Yan He1, Xibin Zhou2, Fajie Yuan2

  • 1School of Medicine, Westlake University, Hangzhou, Zhejiang 310014, China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310014, China; Research Center for Industries of the Future (RCIF), Westlake University, Hangzhou, Zhejiang 310014, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310014, China; Westlake Center for Genome Editing, Westlake Laboratory of Life Sciences and Biomedicine, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang 310024, China.

STAR protocols
|July 13, 2024
PubMed
概括

蛋白质语言模型 (PLMs) 优化蒂胺-DNA-糖酶 (TDG) 以提高基因编辑. 该协议简化了酶工程,通过减少实验力度改善了活性.

关键词:
生物技术和生物工程克里斯普尔是什么意思?克里斯普尔是什么意思?计算机科学 计算机科学

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Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis11:08

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Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
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Proteins are...
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