对Spatholobus suberectus全长转录组的分析确定了一个编码R2R3-MYB转录因子的R2R3-MYB转录因子编码基因SsMYB158,该基因调节着黄类生物合成
Shuangshuang Qin1, Guili Wei1, Quan Lin1
1Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, National Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China; National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, China.
Plant physiology and biochemistry : PPB
|July 13, 2024
概括
研究人员确定了SsMYB158转录因子 (TF) 作为Spatholobus suberectus中黄类生物合成的关键调节者. 过度表达这种TF显著增加了黄类和儿茶素水平,为分子育种铺平了道路.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 基因组学就是基因组学.
背景情况:
- (Leguminosae) 是一种药用植物,其主要生物活性化合物是黄类植物.
- 转录因子 (TFs) 调节S. suberectus中黄类生物合成的精确机制在很大程度上仍未被描述.
研究的目的:
- 鉴定和描述涉及调节Spatholobus suberectus中黄类生物合成的转录因子.
- 阐明SsMYB158转录因子在控制黄酸生产中的作用.
主要方法:
- 使用SMRT测序对S. suberectus进行全长转录组分析.
- 转录因子的识别和表征,包括SsMYB158.8.
- 对SsMYB158在Nicotiana benthamiana中的过度表达和S. suberectus中的过渡性过度表达的研究.
主要成果:
- 转录组分析确定了6781个TF,包括R2R3-MYB类TF,SsMYB158.
- 对SsMYB158的过度表达导致了总黄素和catechin水平的显著增加.
- 关键的黄类生物合成途径基因的表达在SsMYB158过度表达后显著上调.
结论:
- 鉴定SsMYB158是斯帕索洛布斯 (Spatholobus suberectus) 中黄类生物合成的关键积极调节剂.
- 这一发现为S. suberectus的分子育种提供了基础,以提高药物化合物生产.
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