在尼古丁烟草中的尼古丁生物合成调节中,HY5和COP1的作用是对抗性的
Deeksha Singh1, Shambhavi Dwivedi2, Nivedita Singh2
1Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Plant physiology and biochemistry : PPB
|July 13, 2024
概括
烟草中尼古丁生物合成的轻度调节涉及构成性光形生成性1 (COP1) 和延长性低基5 (HY5) 之间的对抗性相互作用. NtCOP1通过抑制NtHY5运输而对尼古丁水平产生负面影响,这对依赖光的积累至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生物化学 植物生物化学
- 光信号通道的光信号通道.
背景情况:
- 尼古丁是Nicotiana物种中的主要化物,生物合成基因主要在根中表达.
- 光线通过发芽到根部的移动因子ELONGATED HYPOCOTYL 5 (HY5) 影响尼古丁的积累.
- 在依赖光的尼古丁积累中,构成性光形致性1 (COP1) 的作用尚不清楚.
研究的目的:
- 研究烟草COP1同类物NtCOP1在调节依赖光的尼古丁生物合成中的作用.
- 阐明NtCOP1与NtHY5相互作用以控制尼古丁水平的分子机制.
主要方法:
- 补充阿拉比多普西斯cop1-4突变体与NtCOP1.1的补充.
- 产生和分析NtCOP1过度表达 (NtCOP1OX) 和CRISPR/Cas9淘汰 (NtCOP1CR) 的烟草植物.
- 酵母两种混合试验,接种实验和西部斑分析以研究蛋白质相互作用和运输.
主要成果:
- NtCOP1成功地补充了阿拉比多普西斯cop1-4突变.
- NtCOP1OX植物显示尼古丁和黄醇含量降低,与降低调节的烯胺路径有关.
- 在NtCOP1CR植物中,尼古丁含量显著增加.
- 发现NtCOP1阻碍了NtHY5从芽到根的运输,从而调节了尼古丁生物合成.
结论:
- NtCOP1在烟草中尼古丁生物合成的光依赖调节中起着至关重要的作用.
- NtCOP1通过抑制其从芽到根的运输来对抗NtHY5的功能,从而控制尼古丁的积累.
- HY5-COP1相互作用是植物光介导代谢途径的关键调节节点.
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