ACADM抑制AMPK激活以调节PEDV诱导的脂质和β-氧化,以损害病毒复制
Quanqiong Wang1, Qi Zhang1, Xiaojie Shi1
1College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
The Journal of biological chemistry
|July 13, 2024
概括
中链乙基-CoA脱酶 (ACADM) 通过调节脂质代谢来抑制猪流行性腹病毒 (PEDV) 复制. 这种酶通过减少脂肪酸氧化来减少病毒复制,提供了一种新的控制策略.
科学领域:
- 病毒学 病毒学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 猪流行性腹病毒 (PEDV) 在猪群中造成重大经济损失. 中链乙基-CoA脱酶 (ACADM) 参与脂质代谢和病原体感染,但其在PEDV复制中的作用尚不清楚.
- 了解宿主-病原体相互作用对于开发有效的抗病毒策略至关重要.
研究的目的:
- 调查ACADM在调节PEDV复制中的作用.
- 阐明ACADM与PEDV相互作用的潜在分子机制.
主要方法:
- 免疫沉质谱测量以确定宿主-病原体相互作用.
- 同免疫沉和激光共聚焦显微镜以确认蛋白质相互作用.
- 在PEDV感染期间分析ACADM表达水平.
- 在ACADM过度表达或敲击后评估病毒复制.
- 研究脂肪酸β-氧化通路及其通过ACADM的调制.
主要成果:
- ACADM被确定为PEDV非结构蛋白4 (NSP4) 的结合伙伴.
- 在PEDV感染期间,ACADM表达增加.
- 过度表达ACADM抑制了PEDV复制,而ACADM倒置则增强了它.
- 抑制脂肪酸β-氧化减少了PEDV复制.
- 通过阻碍AMPK介导的脂质消化,ACADM通过降低细胞自由脂肪酸水平和β-氧化来抑制PEDV复制.
结论:
- ACADM通过调节脂质代谢来负面调节PEDV复制.
- ACADM的机制涉及通过抑制脂质形成来降低脂肪酸β-氧化.
- 这项研究揭示了控制PEDV感染的新型治疗标.
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